Transcript: Human XM_005259327.3

PREDICTED: Homo sapiens BR serine/threonine kinase 1 (BRSK1), transcript variant X2, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
BRSK1 (84446)
Length:
3177
CDS:
617..2683

Additional Resources:

NCBI RefSeq record:
XM_005259327.3
NBCI Gene record:
BRSK1 (84446)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_005259327.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000199927 GCTTAGCAGATCCGGACAGGG pLKO.1 2975 3UTR 100% 0.000 0.000 N BRSK1 n/a
2 TRCN0000355544 CCAACTGTGAATCTGTAAATA pLKO_005 2737 3UTR 100% 15.000 10.500 N BRSK1 n/a
3 TRCN0000218088 ACGTCTACGAGAACAAGAAAT pLKO_005 635 CDS 100% 13.200 9.240 N BRSK1 n/a
4 TRCN0000360985 ACGTCTACGAGAACAAGAAAT pLKO_005 635 CDS 100% 13.200 9.240 N Brsk1 n/a
5 TRCN0000355545 AGGCTCAGTCTGGAGCAAATT pLKO_005 1163 CDS 100% 13.200 9.240 N BRSK1 n/a
6 TRCN0000002399 CCCAACTGTGAATCTGTAAAT pLKO.1 2736 3UTR 100% 13.200 9.240 N BRSK1 n/a
7 TRCN0000078904 CGTCTACGAGAACAAGAAATA pLKO.1 636 CDS 100% 13.200 9.240 N LOC436019 n/a
8 TRCN0000226449 GCGTGTCCAGAGGTGATTAAG pLKO_005 935 CDS 100% 13.200 9.240 N BRSK1 n/a
9 TRCN0000355602 GGACAGACAGGGCTGGTTAAA pLKO_005 469 5UTR 100% 13.200 9.240 N BRSK1 n/a
10 TRCN0000226448 ATCTGCCACAGAGACCTAAAG pLKO_005 800 CDS 100% 10.800 7.560 N BRSK1 n/a
11 TRCN0000194778 CAAATATTCCTCGTGCTAAAG pLKO.1 2165 CDS 100% 10.800 7.560 N BRSK1 n/a
12 TRCN0000002400 CCCACTTCATTCCTCCAGATT pLKO.1 1098 CDS 100% 4.950 3.465 N BRSK1 n/a
13 TRCN0000195522 CGACGTCTACGAGAACAAGAA pLKO.1 633 CDS 100% 4.950 3.465 N BRSK1 n/a
14 TRCN0000226451 ATAAGGCCCAAGGAACATGTC pLKO_005 2755 3UTR 100% 4.050 2.835 N BRSK1 n/a
15 TRCN0000002398 CAGCATCAAAGCAGACATCGT pLKO.1 2200 CDS 100% 2.640 1.848 N BRSK1 n/a
16 TRCN0000078905 CCACTGCATCACGGGTCAGAA pLKO.1 498 5UTR 100% 1.650 1.155 N LOC436019 n/a
17 TRCN0000002397 CAGAAACATCCTTGGTACCTA pLKO.1 1184 CDS 100% 0.000 0.000 N BRSK1 n/a
18 TRCN0000199045 CCCGACGTCCTAGAGAGCATG pLKO.1 1295 CDS 100% 0.000 0.000 N BRSK1 n/a
19 TRCN0000226450 ACAAATATTCCTCGTGCTAAA pLKO_005 2164 CDS 100% 10.800 6.480 N BRSK1 n/a
20 TRCN0000024403 CCTTGGACAAAGAAGAACAAA pLKO.1 2148 CDS 100% 5.625 3.375 N Brsk1 n/a
21 TRCN0000002396 AGCTATTCGACTACCTGGTAA pLKO.1 696 CDS 100% 4.950 2.970 N BRSK1 n/a
22 TRCN0000199415 CAGGGCCCTCTGTCCCTGTGT pLKO.1 2991 3UTR 100% 0.000 0.000 N BRSK1 n/a
23 TRCN0000361048 TCGCCATCCTGAAGCTCATTG pLKO_005 587 5UTR 100% 10.800 6.480 N Brsk1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_005259327.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_15197 pDONR223 0% 68.5% 68.6% None (many diffs) n/a
2 ccsbBroad304_15197 pLX_304 0% 68.5% 68.6% V5 (many diffs) n/a
3 TRCN0000469730 TATCATGTCAACCCTCGCGGGCAG pLX_317 14.2% 68.5% 68.6% V5 (many diffs) n/a
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