Construct: ORF TRCN0000469739

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008209.1_s317c1
Derived from:
ccsbBroadEn_00486
DNA Barcode:
GATTGACCGCAAACCTGATAAGTT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
EFNB2 (1948)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469739
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 1948 EFNB2 ephrin B2 NM_004093.4 100% 100%
2 human 1948 EFNB2 ephrin B2 XM_017020406.2 90.9% 81.5% (many diffs)
3 human 1948 EFNB2 ephrin B2 NM_001372056.1 90.6% 90.3% 406_407ins93
4 human 1948 EFNB2 ephrin B2 NM_001372057.1 88.5% 88.5% 497_498ins114
5 human 1948 EFNB2 ephrin B2 NM_001372058.1 47.8% 44% (many diffs)
6 mouse 13642 Efnb2 ephrin B2 NM_010111.5 92% 95.8% (many diffs)
7 mouse 13642 Efnb2 ephrin B2 XM_011242036.2 85.3% 85.6% (many diffs)
8 mouse 13642 Efnb2 ephrin B2 XM_006508694.3 82.8% 86.3% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1065
ORF length:
999
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggc tgtgagaagg gactccgtgt ggaagtactg ctggggtgtt ttgatggttt
121tatgcagaac tgcgatttcc aaatcgatag ttttagagcc tatctattgg aattcctcga
181actccaaatt tctacctgga caaggactgg tactataccc acagatagga gacaaattgg
241atattatttg ccccaaagtg gactctaaaa ctgttggcca gtatgaatat tataaagttt
301atatggttga taaagaccaa gcagacagat gcactattaa gaaggaaaat acccctctcc
361tcaactgtgc caaaccagac caagatatca aattcaccat caagtttcaa gaattcagcc
421ctaacctctg gggtctagaa tttcagaaga acaaagatta ttacattata tctacatcaa
481atgggtcttt ggagggcctg gataaccagg agggaggggt gtgccagaca agagccatga
541agatcctcat gaaagttgga caagatgcaa gttctgctgg atcaaccagg aataaagatc
601caacaagacg tccagaacta gaagctggta caaatggaag aagttcgaca acaagtccct
661ttgtaaaacc aaatccaggt tctagcacag acggcaacag cgccggacat tcggggaaca
721acatcctcgg ttccgaagtg gccttattTG CAGGGATTGC TTCAGGATGC ATCATCTTCA
781TCGTCATCAT CATCACGCTG GTGGTCCTCT TGCTGAAGTA CCGGAGGAGA CACAGGAAGC
841ACTCGCCGCA GCACACGACC ACGCTGTCGC TCAGCACACT GGCCACACCC AAGCGCAGCG
901GCAACAACAA CGGCTCAGAG CCCAGTGACA TTATCATCCC GCTAAGGACT GCGGACAGCG
961TCTTCTGCCC TCACTACGAG AAGGTCAGCG GGGACTACGG GCACCCGGTG TACATCGTCC
1021AGGAGATGCC CCCGCAGAGC CCGGCGAACA TTTACTACAA GGTCTGCCCA ACTTTCTTGT
1081ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1141AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1201GAAAGGACGA GATTGACCGC AAACCTGATA AGTTACGCGT TAAGTCgaca atcaacctct
1261ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)