Construct: ORF TRCN0000469739
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008209.1_s317c1
- Derived from:
- ccsbBroadEn_00486
- DNA Barcode:
- GATTGACCGCAAACCTGATAAGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EFNB2 (1948)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469739
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1948 | EFNB2 | ephrin B2 | NM_004093.4 | 100% | 100% | |
| 2 | human | 1948 | EFNB2 | ephrin B2 | XM_017020406.2 | 90.9% | 81.5% | (many diffs) |
| 3 | human | 1948 | EFNB2 | ephrin B2 | NM_001372056.1 | 90.6% | 90.3% | 406_407ins93 |
| 4 | human | 1948 | EFNB2 | ephrin B2 | NM_001372057.1 | 88.5% | 88.5% | 497_498ins114 |
| 5 | human | 1948 | EFNB2 | ephrin B2 | NM_001372058.1 | 47.8% | 44% | (many diffs) |
| 6 | mouse | 13642 | Efnb2 | ephrin B2 | NM_010111.5 | 92% | 95.8% | (many diffs) |
| 7 | mouse | 13642 | Efnb2 | ephrin B2 | XM_011242036.2 | 85.3% | 85.6% | (many diffs) |
| 8 | mouse | 13642 | Efnb2 | ephrin B2 | XM_006508694.3 | 82.8% | 86.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1065
- ORF length:
- 999
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtgagaagg gactccgtgt ggaagtactg ctggggtgtt ttgatggttt 121 tatgcagaac tgcgatttcc aaatcgatag ttttagagcc tatctattgg aattcctcga 181 actccaaatt tctacctgga caaggactgg tactataccc acagatagga gacaaattgg 241 atattatttg ccccaaagtg gactctaaaa ctgttggcca gtatgaatat tataaagttt 301 atatggttga taaagaccaa gcagacagat gcactattaa gaaggaaaat acccctctcc 361 tcaactgtgc caaaccagac caagatatca aattcaccat caagtttcaa gaattcagcc 421 ctaacctctg gggtctagaa tttcagaaga acaaagatta ttacattata tctacatcaa 481 atgggtcttt ggagggcctg gataaccagg agggaggggt gtgccagaca agagccatga 541 agatcctcat gaaagttgga caagatgcaa gttctgctgg atcaaccagg aataaagatc 601 caacaagacg tccagaacta gaagctggta caaatggaag aagttcgaca acaagtccct 661 ttgtaaaacc aaatccaggt tctagcacag acggcaacag cgccggacat tcggggaaca 721 acatcctcgg ttccgaagtg gccttattTG CAGGGATTGC TTCAGGATGC ATCATCTTCA 781 TCGTCATCAT CATCACGCTG GTGGTCCTCT TGCTGAAGTA CCGGAGGAGA CACAGGAAGC 841 ACTCGCCGCA GCACACGACC ACGCTGTCGC TCAGCACACT GGCCACACCC AAGCGCAGCG 901 GCAACAACAA CGGCTCAGAG CCCAGTGACA TTATCATCCC GCTAAGGACT GCGGACAGCG 961 TCTTCTGCCC TCACTACGAG AAGGTCAGCG GGGACTACGG GCACCCGGTG TACATCGTCC 1021 AGGAGATGCC CCCGCAGAGC CCGGCGAACA TTTACTACAA GGTCTGCCCA ACTTTCTTGT 1081 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1141 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1201 GAAAGGACGA GATTGACCGC AAACCTGATA AGTTACGCGT TAAGTCgaca atcaacctct 1261 ggattacaaa atttgtgaaa gatt