Construct: ORF TRCN0000469757
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000393.1_s317c1
- Derived from:
- ccsbBroadEn_04009
- DNA Barcode:
- TTAAGGTCAGACGTTCGTGTGGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHID1 (66005)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469757
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 66005 | CHID1 | chitinase domain containing 1 | NM_001142674.2 | 100% | 100% | |
| 2 | human | 66005 | CHID1 | chitinase domain containing 1 | NM_001142675.1 | 100% | 100% | |
| 3 | human | 66005 | CHID1 | chitinase domain containing 1 | NM_023947.4 | 100% | 100% | |
| 4 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_006718282.2 | 100% | 100% | |
| 5 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_017018166.1 | 100% | 100% | |
| 6 | human | 66005 | CHID1 | chitinase domain containing 1 | NM_001142676.2 | 94% | 94% | 110_184del |
| 7 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_005253072.2 | 92.9% | 92.9% | 1_90del |
| 8 | human | 66005 | CHID1 | chitinase domain containing 1 | NM_001142677.2 | 92.1% | 92.1% | 608_609ins93 |
| 9 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_005253073.4 | 92.1% | 92.1% | 608_609ins93 |
| 10 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_024448663.1 | 87.4% | 81.3% | (many diffs) |
| 11 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_011520299.2 | 82.2% | 82.2% | 1_255del |
| 12 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_017018167.2 | 82.1% | 81.1% | (many diffs) |
| 13 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_017018168.1 | 82.1% | 81.1% | (many diffs) |
| 14 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_011520300.2 | 75.7% | 75.7% | 1_255del;863_864ins93 |
| 15 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_011520301.3 | 72.1% | 67.1% | (many diffs) |
| 16 | human | 66005 | CHID1 | chitinase domain containing 1 | XM_011520302.3 | 67.5% | 66.7% | (many diffs) |
| 17 | mouse | 68038 | Chid1 | chitinase domain containing 1 | NM_001142681.1 | 86.2% | 89.1% | (many diffs) |
| 18 | mouse | 68038 | Chid1 | chitinase domain containing 1 | XM_006536230.3 | 79% | 81.3% | (many diffs) |
| 19 | mouse | 68038 | Chid1 | chitinase domain containing 1 | XM_006536231.3 | 74.7% | 74.2% | (many diffs) |
| 20 | mouse | 68038 | Chid1 | chitinase domain containing 1 | XM_011249850.2 | 70.3% | 71.7% | (many diffs) |
| 21 | mouse | 68038 | Chid1 | chitinase domain containing 1 | NM_026522.5 | 68.1% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1245
- ORF length:
- 1179
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gacactcttc aacctcctct ggcttgccct ggcctgcagc cctgttcaca 121 ctaccctgtc aaagtcagat gccaaaaaag ccgcctcaaa gacgctgctg gagaagagtc 181 agttttcaga taagccggtg caagaccggg gtttggtggt gacggacctc aaagctgaga 241 gtgtggttct tgagcatcgc agctactgct cggcaaaggc ccgggacaga cactttgctg 301 gggatgtact gggctatgtc actccatgga acagccatgg ctacgatgtc accaaggtct 361 ttgggagcaa gttcacacag atctcacccg tctggctgca gctgaagaga cgtggccgtg 421 agatgtttga ggtcacgggc ctccacgacg tggaccaagg gtggatgcga gctgtcagga 481 agcatgccaa gggcctgcac atagtgcctc ggctcctgtt tgaggactgg acttacgatg 541 atttccggaa cgtcttagac agtgaggatg agatagagga gctgagcaag accgtggtcc 601 aggtggcaaa gaaccagcat ttcgatggct tcgtggtgga ggtctggaac cagctgctaa 661 gccagaagcg cgtgggcctc atccacatgc tcacccactt ggccgaggct ctgcaccagg 721 cccggctgct ggccctcctg gtcatcccgc ctgccatcac ccccgggacc gaccagctgg 781 gcatgttcac gcacaaggag tttgagcagc tggcccccgt gctggatggt ttcagcctca 841 tgacctacga ctactctaca gcgcatcagc ctggccctaa tgcacccctg tcctgggttc 901 gagccTGCGT CCAGGTCCTG GACCCGAAGT CCAAGTGGCG AAGCAAAATC CTCCTGGGGC 961 TCAACTTCTA TGGTATGGAC TACGCGACCT CCAAGGATGC CCGTGAGCCT GTTGTCGGGG 1021 CCAGGTACAT CCAGACACTG AAGGACCACA GGCCCCGGAT GGTGTGGGAC AGCCAGGCCT 1081 CAGAGCACTT CTTCGAGTAC AAGAAGAGCC GCAGTGGGAG GCACGTCGTC TTCTACCCAA 1141 CCCTGAAGTC CCTGCAGGTG CGGCTGGAGC TGGCCCGGGA GCTGGGCGTT GGGGTCTCTA 1201 TCTGGGAGCT GGGCCAGGGC CTGGACTACT TCTACGACCT GCTCTACCCA ACTTTCTTGT 1261 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 1321 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 1381 GAAAGGACGA TTAAGGTCAG ACGTTCGTGT GGATACGCGT TAAGTCgaca atcaacctct 1441 ggattacaaa atttgtgaaa gatt