Construct: ORF TRCN0000469757

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF000393.1_s317c1
Derived from:
ccsbBroadEn_04009
DNA Barcode:
TTAAGGTCAGACGTTCGTGTGGAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CHID1 (66005)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469757
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 66005 CHID1 chitinase domain containing 1 NM_001142674.2 100% 100%
2 human 66005 CHID1 chitinase domain containing 1 NM_001142675.1 100% 100%
3 human 66005 CHID1 chitinase domain containing 1 NM_023947.4 100% 100%
4 human 66005 CHID1 chitinase domain containing 1 XM_006718282.2 100% 100%
5 human 66005 CHID1 chitinase domain containing 1 XM_017018166.1 100% 100%
6 human 66005 CHID1 chitinase domain containing 1 NM_001142676.2 94% 94% 110_184del
7 human 66005 CHID1 chitinase domain containing 1 XM_005253072.2 92.9% 92.9% 1_90del
8 human 66005 CHID1 chitinase domain containing 1 NM_001142677.2 92.1% 92.1% 608_609ins93
9 human 66005 CHID1 chitinase domain containing 1 XM_005253073.4 92.1% 92.1% 608_609ins93
10 human 66005 CHID1 chitinase domain containing 1 XM_024448663.1 87.4% 81.3% (many diffs)
11 human 66005 CHID1 chitinase domain containing 1 XM_011520299.2 82.2% 82.2% 1_255del
12 human 66005 CHID1 chitinase domain containing 1 XM_017018167.2 82.1% 81.1% (many diffs)
13 human 66005 CHID1 chitinase domain containing 1 XM_017018168.1 82.1% 81.1% (many diffs)
14 human 66005 CHID1 chitinase domain containing 1 XM_011520300.2 75.7% 75.7% 1_255del;863_864ins93
15 human 66005 CHID1 chitinase domain containing 1 XM_011520301.3 72.1% 67.1% (many diffs)
16 human 66005 CHID1 chitinase domain containing 1 XM_011520302.3 67.5% 66.7% (many diffs)
17 mouse 68038 Chid1 chitinase domain containing 1 NM_001142681.1 86.2% 89.1% (many diffs)
18 mouse 68038 Chid1 chitinase domain containing 1 XM_006536230.3 79% 81.3% (many diffs)
19 mouse 68038 Chid1 chitinase domain containing 1 XM_006536231.3 74.7% 74.2% (many diffs)
20 mouse 68038 Chid1 chitinase domain containing 1 XM_011249850.2 70.3% 71.7% (many diffs)
21 mouse 68038 Chid1 chitinase domain containing 1 NM_026522.5 68.1% 69.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1245
ORF length:
1179
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgcg gacactcttc aacctcctct ggcttgccct ggcctgcagc cctgttcaca
121ctaccctgtc aaagtcagat gccaaaaaag ccgcctcaaa gacgctgctg gagaagagtc
181agttttcaga taagccggtg caagaccggg gtttggtggt gacggacctc aaagctgaga
241gtgtggttct tgagcatcgc agctactgct cggcaaaggc ccgggacaga cactttgctg
301gggatgtact gggctatgtc actccatgga acagccatgg ctacgatgtc accaaggtct
361ttgggagcaa gttcacacag atctcacccg tctggctgca gctgaagaga cgtggccgtg
421agatgtttga ggtcacgggc ctccacgacg tggaccaagg gtggatgcga gctgtcagga
481agcatgccaa gggcctgcac atagtgcctc ggctcctgtt tgaggactgg acttacgatg
541atttccggaa cgtcttagac agtgaggatg agatagagga gctgagcaag accgtggtcc
601aggtggcaaa gaaccagcat ttcgatggct tcgtggtgga ggtctggaac cagctgctaa
661gccagaagcg cgtgggcctc atccacatgc tcacccactt ggccgaggct ctgcaccagg
721cccggctgct ggccctcctg gtcatcccgc ctgccatcac ccccgggacc gaccagctgg
781gcatgttcac gcacaaggag tttgagcagc tggcccccgt gctggatggt ttcagcctca
841tgacctacga ctactctaca gcgcatcagc ctggccctaa tgcacccctg tcctgggttc
901gagccTGCGT CCAGGTCCTG GACCCGAAGT CCAAGTGGCG AAGCAAAATC CTCCTGGGGC
961TCAACTTCTA TGGTATGGAC TACGCGACCT CCAAGGATGC CCGTGAGCCT GTTGTCGGGG
1021CCAGGTACAT CCAGACACTG AAGGACCACA GGCCCCGGAT GGTGTGGGAC AGCCAGGCCT
1081CAGAGCACTT CTTCGAGTAC AAGAAGAGCC GCAGTGGGAG GCACGTCGTC TTCTACCCAA
1141CCCTGAAGTC CCTGCAGGTG CGGCTGGAGC TGGCCCGGGA GCTGGGCGTT GGGGTCTCTA
1201TCTGGGAGCT GGGCCAGGGC CTGGACTACT TCTACGACCT GCTCTACCCA ACTTTCTTGT
1261ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT
1321AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG
1381GAAAGGACGA TTAAGGTCAG ACGTTCGTGT GGATACGCGT TAAGTCgaca atcaacctct
1441ggattacaaa atttgtgaaa gatt

Download FASTA (ORF) (Full)