Construct: ORF TRCN0000469759
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001572.1_s317c1
- Derived from:
- ccsbBroadEn_06539
- DNA Barcode:
- ACGTGGAAGTGGTCTCTTTCGTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LSS (4047)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469759
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4047 | LSS | lanosterol synthase | NM_001001438.2 | 99.9% | 99.8% | 864G>C;1924T>G |
| 2 | human | 4047 | LSS | lanosterol synthase | NM_002340.6 | 99.9% | 99.8% | 864G>C;1924T>G |
| 3 | human | 4047 | LSS | lanosterol synthase | NM_001145436.2 | 98.4% | 98.3% | 395_396ins33;831G>C;1891T>G |
| 4 | human | 4047 | LSS | lanosterol synthase | NM_001145437.1 | 88.9% | 88.9% | 0_1ins240;624G>C;1684T>G |
| 5 | mouse | 16987 | Lss | lanosterol synthase | NM_146006.2 | 82.9% | 85.6% | (many diffs) |
| 6 | mouse | 16987 | Lss | lanosterol synthase | XM_006513283.3 | 63.3% | 65% | (many diffs) |
| 7 | mouse | 16987 | Lss | lanosterol synthase | XM_017313817.1 | 58.8% | 60.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2262
- ORF length:
- 2196
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac ggagggcacg tgtctgcggc gccgaggggg cccctacaag accgagcccg 121 ccaccgacct cggccgctgg cgactcaact gcgagagggg ccggcagacg tggacctacc 181 tgcaggacga gcgcgccggc cgcgagcaga ccggcctgga agcctacgcc ctggggctgg 241 acaccaagaa ttactttaag gacttgccca aagcccacac cgcctttgag ggggctctga 301 acgggatgac attttacgtg gggctgcagg ctgaggatgg gcactggacg ggtgattatg 361 gtggcccact tttcctcctg ccaggcctcc tgatcacttg ccacgtggca cgcatccctc 421 tgccagccgg atacagagaa gagattgtgc ggtacctgcg gtcagtgcag ctccctgacg 481 gtggctgggg cctgcacatt gaggataagt ccaccgtgtt tgggactgcg ctcaactatg 541 tgtctctcag aattctgggt gttgggcctg acgatcctga cctggtacga gcccggaaca 601 ttcttcacaa gaaaggtggt gctgtggcca tcccctcctg ggggaagttc tggctggctg 661 tcctgaatgt ttacagctgg gaaggcctca ataccctgtt cccagagatg tggctgtttc 721 ctgactgggc accggcacac ccctccacac tctggtgcca ctgccggcag gtgtacctgc 781 ccatgagcta ctgctacgcc gttcggctga gtgccgcgga agacccgctg gtccagagcc 841 tccgccagga gctctatgtg gaggacttcg ccagcattga ctggctggcg cagaggaaca 901 acgtggcccc cgacgagctg tacacgcccc acagctggct gctccgcgtg gtatatgcgc 961 tcctcaacct gtatgagcac caccacagtg cccacctgcg gcagcgggcc gtgcagaagc 1021 tgtatgaaca cattgtggcc gacgaccgat tcaccaagag catcagcatc ggcccgatct 1081 cgaaaaccat caacatgctt gtgcgctggt atgtggacgg gcccgcctcc actgccttcc 1141 aggagcatgt ctccagaatc ccggactatc tctggatggg ccttgacggc atgaaaatgc 1201 agggcaccaa cggctcacag atctgggaca ccgcattcgc catccaggct ctgcttgagg 1261 cgggcgggca ccacaggccc gagttttcgt cctgcctgca gaaggctcat gagttcctga 1321 ggctctcaca ggtcccagat aaccctcccg actaccagaa gtactaccgc cagatgcgca 1381 agggtggctt ctccttcagt acgctggact gcggctggat cgtttctgac tgcacggctg 1441 aggccttgaa ggctgtgctg ctcctgcagg agaagtgtcc ccatgtcacc gagcacatcc 1501 ccagagaacg gctctgcgat gctgtggctg tgctgctgaa catgagaaat ccagatggag 1561 ggttcgccac ctatgagacc aagcgtgggg ggcacttgct ggagctgctg aacccctcgg 1621 aggtcttcgg ggacatcatg attgactaca cctatgtgga gtgcacctca gccgtgatgc 1681 aggcgcttaa gtatttccac aagcgtttcc cggagcacag ggcagcggag atccgggaga 1741 ccctcacgca gggcttagag ttctgtcggc ggcagcagag ggccgatggc tcctgggaag 1801 gctcctgggg agtttgcttc acctacggca cctggtttgg cctggaggcc TTCGCCTGTA 1861 TGGGGCAGAC CTACCGAGAT GGGACTGCCT GTGCAGAGGT CTCCCGGGCC TGTGACTTCC 1921 TGCTGTCCCG GCAGATGGCA GACGGAGGCT GGGGGGAGGA CTTTGAGTCC TGCGAGGAGC 1981 GGCGTTATGT GCAGAGTGCC CAGTCCCAGA TCCATAACAC ATGCTGGGCC ATGATGGGGC 2041 TGATGGCCGT TCGGCATCCT GACATCGAGG CCCAGGAGAG AGGAGTCCGG TGTCTACTTG 2101 AGAAACAGCT CCCCAATGGC GACTGGCCGC AGGAAAACAT TGCTGGGGTC TTCAACAAGT 2161 CCTGTGCCAT CTCCTACACG AGCTACAGGA ACATCTTCCC CATCTGGGCC CTCGGCCGCT 2221 TCTCCCAGCT GTACCCTGAG AGAGCCCTTG CTGGCCACCC CTGCCCAACT TTCTTGTACA 2281 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 2341 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 2401 AGGACGAACG TGGAAGTGGT CTCTTTCGTC CACGCGTTAA GTCgacaatc aacctctgga 2461 ttacaaaatt tgtgaaagat t