Construct: ORF TRCN0000469761
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000813.1_s317c1
- Derived from:
- ccsbBroadEn_01004
- DNA Barcode:
- GCTGCGCTCAATACTTTACAAGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MELTF (4241)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469761
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4241 | MELTF | melanotransferrin | NM_033316.4 | 100% | 100% | |
| 2 | human | 4241 | MELTF | melanotransferrin | XM_011512851.2 | 42.1% | 36.8% | (many diffs) |
| 3 | human | 4241 | MELTF | melanotransferrin | NM_005929.6 | 38.2% | 33.5% | (many diffs) |
| 4 | human | 4241 | MELTF | melanotransferrin | XM_006713643.3 | 36.9% | 32.4% | (many diffs) |
| 5 | human | 4241 | MELTF | melanotransferrin | XM_011512850.2 | 36.9% | 32.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 972
- ORF length:
- 906
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg gggtccgagc ggggctctgt ggctgctcct ggctctgcgc accgtgctcg 121 gtggcatgga ggtgcggtgg tgcgccacct cggacccaga gcagcacaag tgcggcaaca 181 tgagcgaggc cttccgggaa gcgggcatcc agccctccct cctctgcgtc cggggcacct 241 ccgccgacca ctgcgtccag ctcatcgcgg cccaggaggc tgacgccatc actctggatg 301 gaggagccat ctatgaggcg ggaaaggagc acggcctgaa gccggtggtg ggcgaagtgt 361 acgatcaaga ggtcggtacc tcctattacg ccgtggctgt ggtcaggagg agctcccatg 421 tgaccattga caccctgaaa ggcgtgaagt cctgccacac gggcatcaat cgcacagtgg 481 gctggaacgt gcccgtgggc tacctggtgg agagcggccg cctctcggtg atgggctgcg 541 atgtactcaa agctgtcagc gactattttg ggggcagctg cgtcccgggg gcaggagaga 601 ccagttactc tgagtcccTC TGTCGCCTCT GCAGGGGTGA CAGCTCTGGG GAAGGGGTGT 661 GTGACAAGAG CCCCCTGGAG AGATACTACG ACTACAGCGG GGCCTTCCGG TGCCTGGCGG 721 AAGGGGCAGG GGACGTGGCT TTTGTGAAGC ACAGCACGGT ACTGGAGAAC ACGGATGAAA 781 GTCCATCACG AAGGCAAACA TGGACCAGAT CTGAGGAGGA AGAAGGCGAG TGCCCTGCAC 841 ACGAGGAAGC ACGTAGGACG ATGCGCTCTA GTGCTGGGCA AGCCTGGAAA TGGGCTCCCG 901 TTCACAGGCC CCAGGACGAG TCTGACAAAG GAGAATTTGG AAAACGGGCA AAGAGTAGGG 961 ATATGTTGGG TTACCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 1021 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 1081 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGAGCT GCGCTCAATA CTTTACAAGT 1141 GACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t