Construct: ORF TRCN0000469764
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005894.1_s317c1
- Derived from:
- ccsbBroadEn_05403
- DNA Barcode:
- CCTAGGCTACGAGTTAGAATACTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DTWD2 (285605)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469764
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285605 | DTWD2 | DTW domain containing 2 | NM_173666.4 | 100% | 100% | |
| 2 | human | 285605 | DTWD2 | DTW domain containing 2 | XM_011543338.3 | 93.7% | 93.7% | 595_654del |
| 3 | human | 285605 | DTWD2 | DTW domain containing 2 | NM_001308081.1 | 77% | 75.8% | (many diffs) |
| 4 | human | 285605 | DTWD2 | DTW domain containing 2 | XM_011543340.2 | 72.1% | 71% | (many diffs) |
| 5 | mouse | 68857 | Dtwd2 | DTW domain containing 2 | NM_026854.3 | 85.6% | 84.5% | (many diffs) |
| 6 | mouse | 68857 | Dtwd2 | DTW domain containing 2 | XM_006526212.2 | 46% | 37.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 963
- ORF length:
- 894
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagtcgcag aaagaggcac gaacactcca ggagcccgtt gcgcggcctt 121 ctggggcctc aagctctcag acgccgaacg acaaggagcg gcgggagggc ggcgcagtgc 181 cggcggcggc tgccctgggc gcagaggcgg acgacgacag tgcggacggg ctgtgggagc 241 tgccggtgga gccggccgag cggaggcctg agtgcacccg ctgcagccgg cctcagaaag 301 tgtgtttgtg tccatttctc ccagcgcacc ctctgcatat ctctacccac ttgtacataa 361 ttcagcatcc agcagaggaa aacaaagtgt tgcgtacagt tcctctacta gcagcatgcc 421 tcccccagga caagtgtaaa gtgaagatcg gtcgtcgctt cagtgaagaa agagatcctg 481 aactttcaac tgtttgccgg aagtctggta cattaatatt atatccaggg gctgaagctg 541 ctaatttGGA AGAATTTATA TTAGATTCTC CTGTTTATCC TTCTACAATC ATCATCATTG 601 ATGGTACATG GAGCCAGGCT AAGGACATTT TCTATAAGAA CTCCTTGTTC CGACATCCCA 661 AACAGGTGCA ATTAAAAACT AGCATTTCTA GTCAGTATGT AATTCGGATG CAGCCGACTA 721 ATAGATGCCT TTCTACACTG GAGTGTGCAG CTGTTGCTCT TTCCATCTTG GAGAAAAATA 781 ATTACATACA AGAGACTTTG CTTCGCCCTC TTCAAGCTTT ATGCTCCTTT CAACTTCAGC 841 ATGGTGCCCA AATTCGCCTC AGCAAGGAAC ACCTTCTGAA AAATGGATTA TATCCTAAAC 901 CAATGCCAAA GAACAAACGC AAACTCAGGA AAATGGAATT GTTAATGAAT AGTGTTAAAA 961 TTTTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGACC TAGGCTACGA GTTAGAATAC TGACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt