Construct: ORF TRCN0000469765
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004102.1_s317c1
- Derived from:
- ccsbBroadEn_13183
- DNA Barcode:
- CCTCCGTGTCCGCAAAACTAATGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- B3GAT2 (135152)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469765
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 135152 | B3GAT2 | beta-1,3-glucuronyltransfer... | NM_080742.3 | 77.7% | 77.3% | 374_589del |
| 2 | mouse | 280645 | B3gat2 | beta-1,3-glucuronyltransfer... | NM_172124.2 | 68.6% | 69.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 822
- ORF length:
- 753
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtccgcg cttttcaccc gcttctttat cctcctgccc tggatcctaa 121 ttgtcatcat catgctcgac gtggacacgc gcaggccagt gcccccgctc accccgcgcc 181 cctacttctc tccctacgcg gtgggccgcg ggggcgcccg actcccgctc cgcaggggcg 241 gcccggctca cgggacccaa aagcgcaacc agtctcggcc gcagccacag ccggagccgc 301 agctgcccac catctatgcc atcacgccca ccTACAGCCG CCCGGTGCAG AAAGCGGAGC 361 TGACCCGCCT GGCCAACACG TTCCGCCAGG TGGCGCAGCT GCACTGGATC CTGGTGGAGG 421 ACGCGGCGGC GCGCAGCGAG CAGATGCGAA CCACCCGCAA GGTCTCCGTC TGGCCTGTGG 481 GCCTGGTTGG TGGGCGGCGC TACGAACGTC CGCTGGTGGA AAACGGCAAA GTTGTTGGCT 541 GGTACACCGG CTGGAGAGCA GACAGGCCTT TTGCCATCGA CATGGCAGGA TTTGCTGTAA 601 GTCTTCAAGT CATTTTGTCC AATCCAAAAG CTGTATTTAA GCGTCGTGGA TCCCAGCCAG 661 GGATGCAAGA ATCTGACTTT CTCAAACAGA TAACAACAGT CGAAGAACTG GAACCGAAAG 721 CAAATAACTG CACTAAGGTT CTCGTGTGGC ACACTCGGAC AGAGAAGGTT AATCTAGCCA 781 ACGAGCCAAA GTACCACCTG GACACAGTGA AAATTGAGGT ATTGCCAACT TTCTTGTACA 841 AAGTGGTTGA TATCGGTAAG CCTATCCCTA ACCCTCTCCT CGGTCTCGAT TCTACGTAGT 901 AATGAACTAG TCCGTAACTT GAAAGTATTT CGATTTCTTG GCTTTATATA TCTTGTGGAA 961 AGGACGACCT CCGTGTCCGC AAAACTAATG TACGCGTTAA GTCgacaatc aacctctgga 1021 ttacaaaatt tgtgaaagat t