Construct: ORF TRCN0000469769
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017531.1_s317c1
- Derived from:
- ccsbBroadEn_03456
- DNA Barcode:
- TCGGCTGCCCCAACCTTCTTTAAG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROPN1 (54763)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469769
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317774.1 | 100% | 100% | |
| 2 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_017578.4 | 100% | 100% | |
| 3 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512933.2 | 100% | 100% | |
| 4 | human | 54763 | ROPN1 | rhophilin associated tail p... | XM_011512934.2 | 100% | 100% | |
| 5 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001012337.3 | 97.1% | 95.7% | (many diffs) |
| 6 | human | 152015 | ROPN1B | rhophilin associated tail p... | NM_001308313.2 | 97.1% | 95.7% | (many diffs) |
| 7 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_006713513.3 | 97.1% | 95.7% | (many diffs) |
| 8 | human | 54763 | ROPN1 | rhophilin associated tail p... | NM_001317775.1 | 56.6% | 56.6% | 0_1ins276 |
| 9 | human | 152015 | ROPN1B | rhophilin associated tail p... | XM_005247138.3 | 54.7% | 53.7% | (many diffs) |
| 10 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | NM_030744.2 | 85.5% | 83.4% | (many diffs) |
| 11 | mouse | 76378 | Ropn1 | ropporin, rhophilin associa... | XM_006522698.3 | 85.5% | 83.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 705
- ORF length:
- 636
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctcagaca gataagccaa catgcatccc gccggagctg ccgaagatgc 121 tgaaggagtt tgccaaagcc gccattaggg tgcagccgca ggacctcatc cagtgggcag 181 ccgattattt tgaggccctg tcccgtggag agacgcctcc ggtgagagag cggtctgagc 241 gagtcgcttt gtgtaaccgg gcagagctaa cacctgagct gttaaagaTC CTGCATTCTC 301 AGGTTGCTGG CAGACTGATC ATCCGTGCAG AGGAGCTGGC CCAGATGTGG AAAGTGGTGA 361 ATCTCCCAAC AGATCTGTTT AATAGTGTGA TGAATGTGGG TCGCTTCACG GAGGAGATCG 421 AGTGGCTGAA GTTTTTAGCC CTTGCTTGCA GCGCTCTGGG AGTTACTATT ACCAAAACTC 481 TCAAGATAGT GTGTGAGGTC TTATCATGTG ACCATAATGG TGGGTCGCCC CGGATCCCGT 541 TCAGCACCTT CCAGTTTCTC TACACGTATA TTGCCAAAGT GGATGGGGAG ATCTCTGCAT 601 CACATGTCAG CAGGATGCTA AACTACATGG AACAGGAAGT AATTGGCCCT GATGGTATAA 661 TCACAGTGAA TGACTTTACC CAAAACCCCA GGGTTCAGCT GGAGTTGCCA ACTTTCTTGT 721 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 781 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 841 GAAAGGACGA TCGGCTGCCC CAACCTTCTT TAAGACGCGT TAAGTCgaca atcaacctct 901 ggattacaaa atttgtgaaa gatt