Construct: ORF TRCN0000469775
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008597.1_s317c1
- Derived from:
- ccsbBroadEn_15963
- DNA Barcode:
- GACGGATCCTAGGAAGAACCTATT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- TMBIM1 (64114)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469775
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321427.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 2 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321428.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 3 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321429.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 4 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321430.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 5 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321432.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 6 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321433.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 7 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321435.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 8 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321436.1 | 99.7% | 57.3% | 508_509insT;846C>G |
| 9 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_022152.6 | 99.6% | 57.3% | 237A>G;508_509insT;846C>G |
| 10 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | XM_011511627.1 | 99.6% | 57.3% | 237A>G;508_509insT;846C>G |
| 11 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NM_001321438.1 | 64.1% | 20.1% | (many diffs) |
| 12 | human | 64114 | TMBIM1 | transmembrane BAX inhibitor... | NR_135643.1 | 35.8% | (many diffs) | |
| 13 | mouse | 69660 | Tmbim1 | transmembrane BAX inhibitor... | NM_027154.5 | 84.8% | 53.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 777
- ORF length:
- 711
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgtc caaccccagc gccccaccac catatgaaga ccgcaacccc ctgtacccag 121 gccctccgcc ccctgggggc tatgggcagc catctgtcct gccaggaggg tatcctgcct 181 accctggcta cccgcagcct ggctacggtc accctgctgg ctacccacag cccatgcccc 241 ccacccaccc gatgcccatg aactacggcc caggccatgg ctatgatggg gaggagagag 301 cggtgagtga tagcttcggg cctggagagt gggatgaccg gaaagtgcga cacactttta 361 tccgaaaggt ttactccatc atctccgtgc agctgctcat cactgtggcc atcattgcta 421 tcttcacctt tgtggaacct gtcagcgcct ttgtgaggag aaatgtggct gtctactacg 481 tgtcctatgc tgtcttcgtt gtcacctacc tgatccttgc cTGCTGCCAG GGACCCAGAC 541 GCCGTTTCCC ATGGAACATC ATTCTGCTGA CCCTTTTTTA CTTTTGCCAT GGGCTTCATG 601 ACGGGCACCA TTTCCAGTAT GTACCAAACC AAAGCCGTCA TCATTGCAAT GATCATCACT 661 GCGGTGGTAT CCATTTCAGT CACCATCTTC TGCTTTCAGA CCAAGGTGGA CTTCACCTCG 721 TGCACAGGCC TCTTCTGTGT CCTGGGAATT GTGCTCCTGG TGACTGGGAT TGTCACTAGC 781 ATTGTGCTCT ACTTCCAATA CGTTTACTGG CTCCACATGC TCTATGCTGC TCTGGGGGCC 841 ATTTGTTTCA CCCTGTTCCT GGCTTACGAC ACACAGCTGG TCCTGGGGAA CCGGAAGCAC 901 ACCATCAGCC CGGAGGACTA CATCACTGGC GCCCTGCAGA TTTACACAGA CATCATCTAC 961 ATCTTCACCT TTGTGCTGCA GCTGATGGGG GATCGCAATT ACCCAACTTT CTTGTACAAA 1021 GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGTAGTAA 1081 TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC TTTATATATC TTGTGGAAAG 1141 GACGAGACGG ATCCTAGGAA GAACCTATTA CGCGTTAAGT Cgacaatcaa cctctggatt 1201 acaaaatttg tgaaagatt