Construct: ORF TRCN0000469776
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007696.1_s317c1
- Derived from:
- ccsbBroadEn_11573
- DNA Barcode:
- AAGCTAAAGAAGCTGGACCGACCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KDELR1 (10945)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469776
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10945 | KDELR1 | KDEL endoplasmic reticulum ... | NM_006801.3 | 82.2% | 67.9% | (many diffs) |
| 2 | mouse | 68137 | Kdelr1 | KDEL (Lys-Asp-Glu-Leu) endo... | NM_133950.2 | 74% | 67.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 636
- ORF length:
- 570
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa tctcttccga ttcctgggag acctctccca cctcctcgcc atcatcttgc 121 tactgctcaa aatctggaag tcccgctcgt gcgccGGAAT TTCAGGGAAG AGCCAGGTCC 181 TGTTTGCTGT GGTGTTCACT GCCCGATATC TGGACCTCTT CACCAACTAC ATCTCACTCT 241 ACAACACGTG TATGAAGGTG GTCTACATAG CCTGCTCCTT CACCACGGTC TGGTTGATTT 301 ATAGCAAGTT CAAAGCTACT TACGATGGGA ACCATGACAC GTTCAGAGTG GAGTTCCTGG 361 TCGTTCCCAC AGCCATTCTG GCGTTCCTGG TCAATCATGA CTTCACCCCT CTGGAGATCC 421 TCTGGACCTT CTCCATCTAC CTGGAGTCAG TGGCCATCTT GCCGCAGCTG TTCATGGTGA 481 GCAAGACCGG CGAGGCGGAG ACCATCACCA GCCACTACTT GTTTGTAGTT TTTTGCCTTT 541 TAGACAAGAA AAAAAAATCT TTCCACTCTT TAGTTTTTGA TTCTGATGAC TCGTTTTTCT 601 TCTACTCTGT GGCCCCAATT TTTATAAAGT GTTTTTGCCC AACTTTCTTG TACAAAGTGG 661 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 721 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 781 AAAGCTAAAG AAGCTGGACC GACCCACGCG TTAAGTCgac aatcaacctc tggattacaa 841 aatttgtgaa agatt