Construct: ORF TRCN0000469780
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001926.1_s317c1
- Derived from:
- ccsbBroadEn_07488
- DNA Barcode:
- GCCTTCAGACTATTATAACTATCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHYHIP (9796)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469780
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | NM_001099335.1 | 99.5% | 98.7% | (many diffs) |
| 2 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | NM_001363311.1 | 99.4% | 98.7% | (many diffs) |
| 3 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | NM_001363312.1 | 99.4% | 98.7% | (many diffs) |
| 4 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | NM_014759.5 | 99.4% | 98.7% | (many diffs) |
| 5 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | XM_017014102.2 | 59.2% | 58.7% | (many diffs) |
| 6 | human | 9796 | PHYHIP | phytanoyl-CoA 2-hydroxylase... | NR_156475.1 | 43.5% | (many diffs) | |
| 7 | mouse | 105653 | Phyhip | phytanoyl-CoA hydroxylase i... | NM_145981.3 | 92.4% | 98.1% | (many diffs) |
| 8 | mouse | 105653 | Phyhip | phytanoyl-CoA hydroxylase i... | XM_006518379.3 | 92.4% | 98.1% | (many diffs) |
| 9 | mouse | 105653 | Phyhip | phytanoyl-CoA hydroxylase i... | XM_006518380.3 | 92.4% | 98.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1056
- ORF length:
- 990
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgctgtcc acgccccaca gcattgagat caacaacatc acctgcgact 121 ccttcagcat ctcctgggcc atggaggaca gtgacctgga gagggtcacc cattacttca 181 ttgaccttaa caagaaggag aataagaatt ccaacaagtt caagcaccgg gacgtcccca 241 ccaagctcgt ggccaaggca gtgccgctgc ccatgacggt gagaggccac tggttcctga 301 gcccccgcac ggagtacagt gtggccgtgc agacggcagt gaagcagagc gatggggagt 361 acctggtgtc cggctggagc gagacggtgg agttctgcac tggggattat gccaaggagc 421 acctggctca gcttcaggag aaagctgagc agatcgcagg ccgcatgctc cgcttctccg 481 tcttctaccg caaccatcac aaggagtact tcgagcatgc caggacccac tgcgggaaca 541 tgctgcagcc ttacctgaag gacaacagcg gcagccacgg ctcccccacc agcggtatgc 601 tccacggggt cttcttcagc tgcaacacgg agttcaacac gggccagccc ccgcaggact 661 ccccctacgg ccgctggcgc ttccagatcc cagctcagcg cctcttcaat cccagcacca 721 acctctactt tgcggacttc TACTGCATGT ACACGGCCTA CCACTACGCC ATCCTGGTGC 781 TGGCGCCCAA AGGCTCCATG GGGGACCGCT TCTGCCGCGA CCGCCGGCCC CTCCTGGACA 841 TTGCTTGCAA CAAGTTCCTG ACCTGCAGCG TGGAGGATGG GGAGCTGGTC TTCCGCCACG 901 CCCAGGACCT CATCCTGGAG ATCATCTACA CTGAGCCCGT CGACCTGTCC CTGGGCACCC 961 TGGGGGAGAT CAGTGGGCAC CAGCTCATGA GTCTGTCTAC TGCCGATGCC AAGAAGGACC 1021 CCAGCTGCAA GACCTGCAAC ATCAGCGTGG GCCGCTACCC AACTTTCTTG TACAAAGTGG 1081 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1141 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1201 AGCCTTCAGA CTATTATAAC TATCCACGCG TTAAGTCgac aatcaacctc tggattacaa 1261 aatttgtgaa agatt