Construct: ORF TRCN0000469786
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012993.1_s317c1
- Derived from:
- ccsbBroadEn_01361
- DNA Barcode:
- TTTAGGCAAAAAATAACACCATGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAB27A (5873)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469786
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | NM_004580.5 | 100% | 100% | |
| 2 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | NM_183234.2 | 100% | 100% | |
| 3 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | NM_183235.3 | 100% | 100% | |
| 4 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | NM_183236.3 | 100% | 100% | |
| 5 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_005254576.5 | 100% | 100% | |
| 6 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_011521852.1 | 100% | 100% | |
| 7 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_011521854.1 | 100% | 100% | |
| 8 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_011521855.3 | 100% | 100% | |
| 9 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_011521856.2 | 100% | 100% | |
| 10 | human | 5873 | RAB27A | RAB27A, member RAS oncogene... | XM_024450009.1 | 100% | 100% | |
| 11 | mouse | 11891 | Rab27a | RAB27A, member RAS oncogene... | NM_001301230.1 | 88% | 95.9% | (many diffs) |
| 12 | mouse | 11891 | Rab27a | RAB27A, member RAS oncogene... | NM_001301232.1 | 88% | 95.9% | (many diffs) |
| 13 | mouse | 11891 | Rab27a | RAB27A, member RAS oncogene... | NM_023635.6 | 88% | 95.9% | (many diffs) |
| 14 | mouse | 11891 | Rab27a | RAB27A, member RAS oncogene... | XM_006510780.3 | 88% | 95.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 732
- ORF length:
- 663
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gtctgatgga gattatgatt acctcatcaa gtttttagct ttgggagact 121 ctggtgtagg gaagaccagt gtactttacc aatatacaga tggtaaattt aactccaaat 181 ttatcacaac agtgggcatt gatttcaggg aaaaaagagt ggtgtacaga gccagtgggc 241 cggatggagc cactggcaga ggccagagaa tccacctgca gttatgggac acagcagggc 301 aggagaggtt tcgtagctta acgacagcgt tcttcagaga tgctatgggt tttcttcTAC 361 TTTTTGATCT GACAAATGAG CAAAGTTTCC TCAATGTCAG AAACTGGATA AGCCAGCTAC 421 AGATGCATGC ATATTGTGAA AACCCAGATA TAGTGCTGTG TGGAAACAAG AGTGATCTGG 481 AGGACCAGAG AGTAGTGAAA GAGGAGGAAG CCATAGCACT CGCAGAGAAA TATGGAATCC 541 CCTACTTTGA AACTAGTGCT GCCAATGGGA CAAACATAAG CCAAGCAATT GAGATGCTTC 601 TGGACCTGAT AATGAAGCGA ATGGAACGGT GTGTGGACAA GTCCTGGATT CCTGAAGGAG 661 TGGTGCGATC AAATGGTCAT GCCTCTACGG ATCAGTTAAG TGAAGAAAAG GAGAAAGGGG 721 CATGTGGCTG TTTGCCAACT TTCTTGTACA AAGTGGTTGA TATCGGTAAG CCTATCCCTA 781 ACCCTCTCCT CGGTCTCGAT TCTACGTAGT AATGAACTAG TCCGTAACTT GAAAGTATTT 841 CGATTTCTTG GCTTTATATA TCTTGTGGAA AGGACGATTT AGGCAAAAAA TAACACCATG 901 AACGCGTTAA GTCgacaatc aacctctgga ttacaaaatt tgtgaaagat t