Construct: ORF TRCN0000469788
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004818.1_s317c1
- Derived from:
- ccsbBroadEn_13142
- DNA Barcode:
- TACCACTGTTAAGACACATGGTGA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PPP1R21 (129285)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469788
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_001193475.1 | 49.1% | 49% | 1089_2214delinsG |
| 2 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_152994.5 | 47.1% | 47% | 1089_2307delinsG |
| 3 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NM_001135629.3 | 46.4% | 46.4% | 1089_2340delinsG |
| 4 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XR_001738609.1 | 33.8% | 1_153del;1242_3211delinsG | |
| 5 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | NR_024188.3 | 33.8% | 1_188del;313_351del;1316_3212delinsG | |
| 6 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XR_001738608.1 | 33.1% | 1_153del;1242_3278delinsG | |
| 7 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XM_017003302.1 | 25.7% | 25.6% | 0_1ins495;594_1812delinsG |
| 8 | human | 129285 | PPP1R21 | protein phosphatase 1 regul... | XM_017003301.1 | 25.3% | 25.2% | 0_1ins495;594_1845delinsG |
| 9 | mouse | 73825 | Ppp1r21 | protein phosphatase 1, regu... | NM_028658.4 | 41.8% | 44.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1155
- ORF length:
- 1089
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ctcggctgag ttgcagggga agtaccagaa gctggctcag gagtactcga 121 agcttcgggc tcagaatcag gttctgaaaa aaggtgttgt ggatgaacaa gcaaattctg 181 cagctttaaa ggagcaactg aaaatgaagg atcagtcatt gagaaaacta caacaggaaa 241 tggacagttt gacatttcga aatctgcagc ttgccaagag ggtagaacta cttcaagatg 301 aactagctct aagtgaacca cgaggcaaga aaaacaagaa aagtggagaa tcttcttctc 361 agttgagtca agagcagaag agtgtctttg atgaagatct gcaaaagaag atagaagaga 421 atgaacggtt gcatatacaa ttttttgaag ctgatgagca gcacaagcat gtggaagcag 481 agctgaggag tcgactggcc actctggaga cagaagcagc ccagcaccaa gctgtggttg 541 acggtctcac ccggaagtac atggaaacca ttgagaagct gcagaacgac aaggctaaac 601 tagaagtgaa atctcagact ctagaaaagg aagccaagga atgtcgactt cgaacggaag 661 aatgtcaatt acagttaaag actcttcatg aagatttgtc aggtagatta gaggaatcct 721 tatcaatcat caatgaaaaa gtacctttta atgatacaaa atatagtcag tacaacgctc 781 tgaacgttcc actccaCAAT AGGAGACACC AGCTGAAGAT GCGAGATATT GCTGGGCAGG 841 CCCTGGCTTT TGTTCAGGAT CTTGTGACGG CTCTTCTAAA CTTTCATACC TACACAGAAC 901 AGAGGATTCA AATTTTTCCT GTTGATTCTG CCATTGACAC TATATCTCCA TTGAATCAGA 961 AGTTCTCACA ATACCTTCAT GAAAATGCGT CCTATGTCCG CCCTCTTGAG GAAGGAATGC 1021 TTCATTTATT TGAAAGTATC ACTGAGGATA CTGTGACTGT CTTGGAGACA ACTGTGAAAT 1081 TGAAAACTTT TTCAGAACAC TTAACCTCCT ACATATGTTT TCTTAGGAAG ATTCTTCCCT 1141 ATCAGTTAAA AAGGTACCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 1201 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGAAAGTA 1261 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TACCACTGTT AAGACACATG 1321 GTGAACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt