Construct: ORF TRCN0000469812
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012341.1_s317c1
- Derived from:
- ccsbBroadEn_04960
- DNA Barcode:
- CGGAGCATGTTTCTCCCACCTGAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DUSP19 (142679)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469812
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_080876.4 | 100% | 100% | |
| 2 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_001142314.2 | 76.4% | 76.4% | 273_274ins153 |
| 3 | human | 142679 | DUSP19 | dual specificity phosphatas... | NM_001321519.2 | 55.7% | 42.9% | 271_272ins118;363_364ins170 |
| 4 | human | 142679 | DUSP19 | dual specificity phosphatas... | NR_135688.2 | 11.6% | 1_187del;411_412ins47;792_5144del | |
| 5 | mouse | 68082 | Dusp19 | dual specificity phosphatas... | NM_024438.4 | 84.4% | 80.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 717
- ORF length:
- 651
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgta ctcccttaac caggaaatta aagcattctc ccggaataat ctcaggaagc 121 aatgcaccag ggtgacaacg ctaactggaa agaaaattat agaaacatgg aaagatgcca 181 gaattcatgt tgtggaagaa gtagagccga gcagtggggg tggttgtggt tatgtgcagg 241 accttagctc ggacctgcaa gttGGCGTTA TTAAGCCATG GTTGCTCCTA GGGTCACAAG 301 ATGCTGCTCA TGATTTGGAT ACACTGAAAA AGAATAAGGT GACTCATATT CTTAATGTTG 361 CATATGGAGT TGAAAATGCT TTCCTCAGTG ACTTTACATA TAAGAGCATT TCTATATTGG 421 ATCTGCCTGA AACCAACATC CTGTCTTATT TTCCAGAATG TTTTGAATTT ATTGAAGAAG 481 CAAAAAGAAA AGATGGAGTG GTTCTTGTTC ATTGTAATGC AGGCGTTTCC AGGGCTGCTG 541 CAATTGTAAT AGGTTTCCTG ATGAATTCTG AACAAACCTC ATTTACCAGT GCTTTTTCTT 601 TGGTGAAAAA TGCAAGACCT TCCATATGTC CAAATTCTGG CTTCATGGAG CAGCTTCGTA 661 CATATCAAGA GGGCAAAGAA AGCAATAAGT GTGACAGAAT ACAGGAGAAC AGTTCATGCC 721 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 781 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 841 ATATATCTTG TGGAAAGGAC GACGGAGCAT GTTTCTCCCA CCTGATACGC GTTAAGTCga 901 caatcaacct ctggattaca aaatttgtga aagatt