Construct: ORF TRCN0000469819
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015719.1_s317c1
- Derived from:
- ccsbBroadEn_05471
- DNA Barcode:
- GGACCCCGGTCGTTACTTTTCCAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ABCB5 (340273)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469819
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 340273 | ABCB5 | ATP binding cassette subfam... | NM_178559.6 | 100% | 100% | |
| 2 | human | 340273 | ABCB5 | ATP binding cassette subfam... | XM_011515367.2 | 100% | 100% | |
| 3 | human | 340273 | ABCB5 | ATP binding cassette subfam... | NM_001163941.2 | 64.5% | 64.5% | 1_1335del |
| 4 | human | 340273 | ABCB5 | ATP binding cassette subfam... | NM_001163942.2 | 15.9% | 15.2% | (many diffs) |
| 5 | human | 340273 | ABCB5 | ATP binding cassette subfam... | NM_001163993.3 | 15.4% | 15.2% | 371_372insGGC;374_375delAAinsGC;378_379ins2055 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2505
- ORF length:
- 2436
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggtggatgag aatgacatca gagctttaaa tgtgcggcat tatcgagacc 121 atattggagt ggttagtcaa gagcctgttt tgttcgggac caccatcagt aacaatatca 181 agtatggacg agatgatgtg actgatgaag agatggagag agcagcaagg gaagcaaatg 241 cgtatgattt tatcatggag tttcctaata aatttaatac attggtaggg gaaaaaggag 301 ctcaaatgag tggagggcag aaacagagga tcgcaattgc tcgtgcctta gttcgaaacc 361 ccaagattct gattttagat gaggctacgt ctgccctgga ttcagaaagc aagtcagctg 421 ttcaagctgc actggagaag gcgagcaaag gtcggactac aatcgtggta gcacaccgac 481 tttctactat tcgaagtgca gatttgattg tgaccctaaa ggatggaatg ctggcggaga 541 aaggagcaca tgctgaacta atggcaaaac gaggtctata ttattcactt gtgatgtcac 601 aggatattaa aaaagctgat gaacagatgg agtcaatgac atattctact gaaagaaaga 661 ccaactcact tcctctgcac tctgtgaaga gcatcaagtc agacttcatt gacaaggctg 721 aggaatccac ccaatctaaa gagataagtc ttcctgaagt ctctctatta aaaattttaa 781 agttaaacaa gcctgaatgg ccttttgtgg ttctggggac attggcttct gttctaaatg 841 gaactgttca tccagtattt tccatcatct ttgcaaaaat tataaccatg tttggaaata 901 atgataaaac cacattaaag catgatgcag aaatttattc catgatattc gtcattttgg 961 gtgttatttg ctttgtcagt tatttcatgc agggattatt ttacggcaga gcaggggaaa 1021 ttttaacgat gagattaaga cacttggcct tcaaagccat gttatatcag gatattgcct 1081 ggtttgatga aaaggaaaac agcacaggag gcttgacaac aatattagcc atagatatag 1141 cacaaattca aggagcaaca ggttccagga ttggcgtctt aacacaaaat gcaactaaca 1201 tgggactttc agttatcatt tcctttatat atggatggga gatgacattc ctgattctga 1261 gtattgctcc agtacttgcc gtgacaggaa tgattgaaac cgcagcaatg actggatttg 1321 ccaacaaaga taagcaagaa cttaagcatg ctggaaagat agcaactgaa gctttggaga 1381 atatacgtac tatagtgtca ttaacaaggg aaaaagcctt cgagcaaatg tatgaagaga 1441 tgcttcagac tcaacacaga aatacctcga agaaagcaca gattattgga agctgttatg 1501 cattcagcca tgcctttata tattttgcct atgcggcagg gtttcgattt ggagcctatt 1561 taattcaagc tggacgaatg accccagagg gcatgttcat agtttttact gcaattgcat 1621 atggagctat ggccatcgga gaaacgctcg ttttggctcc tgaatattcc aaagccaaat 1681 cgggggctgc gcatctgttt gccttgttgg aaaagaaacc aaatatagac agccgcagtc 1741 aagaagggaa aaagccagac acatgtgaag ggaatttaga gtttcgagaa gtctctttct 1801 tctatccatg tcgcccagat gttttcatcc tccgtggctt atccctcagt attgagcgag 1861 gaaagacagt agcatttgtg gggagcagcg gctgtgggaa aagcacttct gttcaacttc 1921 tgcagagact ttatgacccc gtgcaaggac aagtgctgtt tgatggtgtg gatgcaaaag 1981 aattgaatgt acagtggctc cgttcccaaa tagcaatcgt tccTCAAGAG CCTGTGCTCT 2041 TCAACTGCAG CATTGCTGAG AACATCGCCT ATGGTGACAA CAGCCGTGTG GTGCCATTAG 2101 ATGAGATCAA AGAAGCCGCA AATGCAGCAA ATATCCATTC TTTTATTGAA GGTCTCCCTG 2161 AGAAATACAA CACACAAGTT GGACTGAAAG GAGCACAGCT TTCTGGCGGC CAGAAACAAA 2221 GACTAGCTAT TGCAAGGGCT CTTCTCCAAA AACCCAAAAT TTTATTGTTG GATGAGGCCA 2281 CTTCAGCCCT CGATAATGAC AGTGAGAAGG TGGTTCAGCA TGCCCTTGAT AAAGCCAGGA 2341 CGGGAAGGAC ATGCCTAGTG GTCACTCACA GGCTCTCTGC AATTCAGAAC GCAGATTTGA 2401 TAGTGGTTCT GCACAATGGA AAGATAAAGG AACAAGGAAC TCATCAAGAG CTCCTGAGAA 2461 ATCGAGACAT ATATTTTAAG TTAGTGAATG CACAGTCAGT GCAGTTGCCA ACTTTCTTGT 2521 ACAAAGTGGT TGATATCGGT AAGCCTATCC CTAACCCTCT CCTCGGTCTC GATTCTACGT 2581 AGTAATGAAC TAGTCCGTAA CTTGAAAGTA TTTCGATTTC TTGGCTTTAT ATATCTTGTG 2641 GAAAGGACGA GGACCCCGGT CGTTACTTTT CCAAACGCGT TAAGTCgaca atcaacctct 2701 ggattacaaa atttgtgaaa gatt