Construct: ORF TRCN0000469825
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017086.1_s317c1
- Derived from:
- ccsbBroadEn_09050
- DNA Barcode:
- CCAGTTAGTCTTTGTTTATCATAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZNF436 (80818)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469825
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80818 | ZNF436 | zinc finger protein 436 | NM_001077195.2 | 99.9% | 100% | 942T>C |
| 2 | human | 80818 | ZNF436 | zinc finger protein 436 | NM_030634.2 | 99.9% | 100% | 942T>C |
| 3 | human | 80818 | ZNF436 | zinc finger protein 436 | NM_001370652.1 | 96% | 96.1% | 0_1ins54;888T>C |
| 4 | mouse | 22704 | Zfp46 | zinc finger protein 46 | XM_006538728.1 | 82.8% | 94.4% | (many diffs) |
| 5 | mouse | 22704 | Zfp46 | zinc finger protein 46 | XM_006538729.1 | 82.8% | 94.4% | (many diffs) |
| 6 | mouse | 22704 | Zfp46 | zinc finger protein 46 | XM_011250229.1 | 82.8% | 94.4% | (many diffs) |
| 7 | mouse | 22704 | Zfp46 | zinc finger protein 46 | NM_009557.3 | 79.4% | 91% | (many diffs) |
| 8 | mouse | 22704 | Zfp46 | zinc finger protein 46 | XM_006538731.1 | 79.4% | 91% | (many diffs) |
| 9 | mouse | 22704 | Zfp46 | zinc finger protein 46 | XM_006538732.1 | 79.4% | 91% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1476
- ORF length:
- 1410
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agccaccctg ctcatggctg ggtcccaggc acctgtgacg tttgaagata 121 tggccatgta tctcacccgg gaagaatgga gacctctgga cgctgcacag agggaccttt 181 accgggatgt tatgcaggag aattatggaa atgttgtctc actagatttt gagatcagga 241 gtgagaacga ggtaaatccc aagcaagaga ttagtgaaga tgtacaattt gggactacat 301 ctgaaagacc tgctgagaat gctgaggaaa atcctgaaag tgaagagggc tttgaaagcg 361 gagataggtc agaaagacaa tggggagatt taacagcaga agagtgggta agctatcctc 421 tccaaccagt cactgatcta cttgtccaca aagaagtcca cacaggcatc cgctatcata 481 tatgttctca ttgtggaaag gccttcagtc agatctcaga ccttaatcga catcagaaga 541 cccacactgg agacagaccc tataaatgtt atgaatgtgg aaaaggcttc agtcgcagct 601 cacaccttat tcagcatcaa agaacacata ctggggagag gccttatgac tgtaacgagt 661 gtgggaaaag ttttggaaga agttctcacc tgattcagca tcagacaatc cacactggag 721 agaagcctca caaatgtaat gagtgtggaa aaagtttctg ccgtctctct cacctaatcc 781 aacaccaaag gacccacagt ggtgagaaac cctatgagtg tgaggagtgt gggaaaagct 841 tcagccggag ctctcaccta gctcagcacc agaggaccca cacgggtgag aaaccttatg 901 aatgtaacga atgtggccga ggcttcagtg agagatctga tctcatcaaa cactatcgag 961 tccacacagg ggagaggccc TACAAGTGTG ATGAGTGTGG GAAGAACTTC AGTCAGAACT 1021 CCGACCTTGT GCGTCATCGC AGAGCCCACA CGGGAGAGAA GCCATACCAC TGTAACGAAT 1081 GTGGGGAAAA TTTCAGCCGC ATCTCACACT TGGTTCAGCA CCAGAGAACT CACACTGGAG 1141 AGAAGCCATA TGAATGCAAT GCTTGTGGGA AAAGCTTCAG CCGGAGCTCT CATCTCATCA 1201 CACACCAGAA AATTCACACT GGAGAGAAGC CTTATGAGTG TAATGAGTGT TGGCGAAGCT 1261 TTGGTGAAAG GTCAGATCTA ATTAAACATC AGAGAACCCA CACAGGGGAG AAGCCCTACG 1321 AGTGTGTGCA GTGTGGGAAA GGTTTCACCC AGAGCTCCAA CCTCATCACA CATCAAAGAG 1381 TTCACACGGG AGAGAAACCT TATGAATGTA CCGAATGTGA GAAGAGTTTC AGCAGGAGCT 1441 CAGCTCTTAT TAAACATAAG AGAGTTCATA CGGACTACCC AACTTTCTTG TACAAAGTGG 1501 TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA 1561 CTAGTCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG 1621 ACCAGTTAGT CTTTGTTTAT CATATACGCG TTAAGTCgac aatcaacctc tggattacaa 1681 aatttgtgaa agatt