Construct: ORF TRCN0000469837
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006544.1_s317c1
- Derived from:
- ccsbBroadEn_03273
- DNA Barcode:
- ATGAATTGTCTACTAAATAAACTA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- THEG (51298)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469837
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51298 | THEG | theg spermatid protein | NM_016585.5 | 100% | 100% | |
| 2 | human | 51298 | THEG | theg spermatid protein | NM_199202.3 | 93.6% | 93.6% | 430_431ins72 |
| 3 | human | 51298 | THEG | theg spermatid protein | XM_011528049.2 | 84.7% | 84.7% | 1_108del;1022_1117del |
| 4 | human | 51298 | THEG | theg spermatid protein | XM_011528050.2 | 79.4% | 79.4% | 1_108del;538_539ins72;950_1045del |
| 5 | human | 51298 | THEG | theg spermatid protein | XM_024451532.1 | 74.9% | 74.9% | 1_108del;535_536ins204 |
| 6 | human | 51298 | THEG | theg spermatid protein | XM_011528051.2 | 69.5% | 69.5% | 1_108del;535_536ins204;818_913del |
| 7 | human | 51298 | THEG | theg spermatid protein | XM_024451533.1 | 60.7% | 37.6% | 1_108del;537_538ins322;864_865ins59 |
| 8 | human | 51298 | THEG | theg spermatid protein | XM_011528052.2 | 51.2% | 50.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1203
- ORF length:
- 1137
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggacagcagg cgaaggtcac tcgggaacca gcccagctct gaggctgcgg 121 gcaggtcgga aagggagcag gacggcgacc cccgtggcct ccagagctct gtgtacgaga 181 gccggcgggt cacagacccc gaacgccagg acctggacaa tgcagagctg ggaccagaag 241 acccagaaga ggagcttccc cccgaggagg tggccgggga ggagttcccg gagaccctgg 301 atcccaaaga ggcactttct gagttggaga gagtcctgga caaggacttg gaagaggaca 361 ttcctgaaat cagccggctg tccatcagcc agaagctccc cagcaccacc atgaccaaag 421 caaggaagag gaggaggcgg aggaggctca tggagctggc agagcccaag ataaactggc 481 aagtcctgaa agacaggaag ggacgctgtg gtaaggggta tgcctggatc tccccatgta 541 agatgagctt gcacttctgt ctctgctggc cctctgtgta ctggaccgag cggttccttg 601 aggacaccac cctcaccatc acagtgcccg cggtgtcccg ccgcgtggag gaactgtctc 661 ggcccaagag attctacctg gaatattaca acaacaacag gacgactcct gtctggccca 721 ttcctcggtc ctccctggaa tacagagcgt cgagtcgcct gaaggaactg gccgccccga 781 agattcgtga taacttctgg agcatgccca tgtctgaggt gtcccaggta tccagggcag 841 cccaaatggc agtccccagc tcgcggatcc tccagttgtc aaagccgaag gccccagcca 901 ccctcttgga agagtgggac cccgtgccaa aacccaagcc acatgtgtca gaccataacc 961 gcctccttca cttggccagg cccaaagctc agtcggacaa gtgcgttcct gaccgagatc 1021 ctcgctggga ggtgctggat gtcaccaaga aggtggtggc cagcccccgg atcatctccc 1081 tggccaagcc caaagtgcgc aagggcctca acgagggata cgacaggcgT CCCCTCGCCT 1141 CTATGAGCTT GCCACCCCCA AAAGCATCAC CAGAAAAGTG TGATCAACCC AGGCCTGGCC 1201 TCTACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1261 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1321 GGCTTTATAT ATCTTGTGGA AAGGACGAAT GAATTGTCTA CTAAATAAAC TAACGCGTTA 1381 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt