Construct: ORF TRCN0000469838
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013877.1_s317c1
- Derived from:
- ccsbBroadEn_08491
- DNA Barcode:
- TATTCTACATGCCATGTGTGGCCG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLPH3L (55204)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469838
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55204 | GOLPH3L | golgi phosphoprotein 3 like | NM_018178.6 | 99.8% | 99.6% | 13A>T |
| 2 | human | 55204 | GOLPH3L | golgi phosphoprotein 3 like | XM_006711428.2 | 95.2% | 94.9% | 1_42del;55A>T |
| 3 | mouse | 229593 | Golph3l | golgi phosphoprotein 3-like | NM_001177669.1 | 75.4% | 76.4% | (many diffs) |
| 4 | mouse | 229593 | Golph3l | golgi phosphoprotein 3-like | NM_146133.3 | 74.3% | 78.7% | (many diffs) |
| 5 | mouse | 229593 | Golph3l | golgi phosphoprotein 3-like | NM_001177670.1 | 56.5% | 60% | (many diffs) |
| 6 | mouse | 229593 | Golph3l | golgi phosphoprotein 3-like | XM_017319544.2 | 30.6% | 28.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 921
- ORF length:
- 855
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac cactttatct caccgggccc gtcgcactga aataagcaag aactctgaaa 121 agaagatgga aagtgaggaa gacagtaatt gggagaaaag tccagacaat gaagattctg 181 gagactctaa ggatatccgc cttactctta tggaagaagt attgcttctg ggactaaaag 241 ataaagaggg gtacacatct ttctggaatg actgcatatc atcaggcctg cgagggggca 301 tcctgataga gctggccatg cggggtcgaa tctatctgga acccccgacc atgcgtaaga 361 agcgactact agacagaaag gtactgctaa agtcagacag cccaacaggt gatgttttac 421 tggatgaaac tctgaaacac atcaaagcaa ctgaacccac agaaactgtc caaacatgga 481 tagagctact cactggtgag acctggaacc ccttcaaatt acagtaccag ctgagaaatg 541 tacgagagcg catcgcaaag aacctagtag agaaaggtat tctaaccact gagaagcaga 601 atttcctgct atttgacatg actactcatc cagtgaccaa tacaacagag aaacagcgac 661 tagtgaaaaa acttcaagat agtgtactag agcggtgggt aaatgaccct cagcgtatgg 721 acaagcgaac aCTAGCACTC CTGGTGCTAG CCCACTCCTC TGATGTGCTA GAGAATGTCT 781 TCTCCTCTCT GACAGATGAC AAGTATGATG TGGCAATGAA TCGAGCCAAG GACTTAGTAG 841 AACTGGACCC TGAAGTGGAA GGGACAAAGC CTAGTGCCAC AGAAATGATC TGGGCTGTGC 901 TGGCAGCCTT CAATAAATCT TACCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 961 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGC CCGTAACTTG 1021 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGATATT CTACATGCCA 1081 TGTGTGGCCG ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt