Construct: ORF TRCN0000469847
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002732.1_s317c1
- Derived from:
- ccsbBroadEn_03474
- DNA Barcode:
- GAGTAGGCTCCAAAGGCCGCCGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PLPPR1 (54886)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469847
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 54886 | PLPPR1 | phospholipid phosphatase re... | NM_017753.3 | 100% | 100% | |
| 2 | human | 54886 | PLPPR1 | phospholipid phosphatase re... | NM_207299.2 | 100% | 100% | |
| 3 | mouse | 272031 | Plppr1 | phospholipid phosphatase re... | NM_178756.4 | 90.6% | 96.3% | (many diffs) |
| 4 | mouse | 272031 | Plppr1 | phospholipid phosphatase re... | XM_006537968.3 | 90.6% | 96.3% | (many diffs) |
| 5 | mouse | 272031 | Plppr1 | phospholipid phosphatase re... | XM_011250049.2 | 90.6% | 96.3% | (many diffs) |
| 6 | mouse | 272031 | Plppr1 | phospholipid phosphatase re... | XM_017320250.1 | 90.6% | 96.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc tgtaggaaac aacactcaac gaagttattc catcatcccg tgttttatat 121 ttgttgagct tgtcatcatg gctgggacag tgctgcttgc ctactacttc gaatgcactg 181 acacttttca ggtgcatatc caaggattct tctgtcagga cggagactta atgaagcctt 241 acccagggac agaggaagaa agcttcatca cccctctggt gctctattgt gtgctggctg 301 ccaccccaac tgctattatt tttattggtg agatatccat gtatttcata aaatcaacaa 361 gagaatccct gattgctcag gagaaaacaa ttctgaccgg agaatgctgt tacctgaacc 421 ccttacttcg aaggatcata agattcacag gggtgtttgc atttggactt tttgctactg 481 acatttttgt aaacgccgga caagtggtca ctgggcactt aacgccatac ttcctgactg 541 tgtgcaagcc aaactacacc agtgcagact gccaagcgca ccaccagttt ataaacaatg 601 ggaacatttg tactggggac ctggaagtga tagaaaaggc tcggagatcc tttccctcca 661 aacacgctgc tctgagcatt tactccgccT TATATGCCAC GATGTATATT ACAAGCACAA 721 TCAAGACGAA GAGCAGTCGA CTGGCCAAGC CGGTGCTGTG CCTCGGAACT CTCTGCACAG 781 CCTTCCTGAC AGGCCTCAAC CGGGTCTCTG AGTATCGGAA CCACTGCTCG GACGTGATTG 841 CTGGTTTCAT CCTGGGCACT GCAGTGGCCC TGTTTCTGGG AATGTGTGTG GTTCATAACT 901 TTAAAGGAAC GCAAGGATCT CCTTCCAAAC CCAAGCCTGA GGATCCCCGT GGAGTACCCC 961 TAATGGCTTT CCCAAGGATA GAAAGCCCTC TGGAAACCTT AAGTGCACAG AATCACTCTG 1021 CGTCCATGAC CGAAGTTACC TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGAGAGT AGGCTCCAAA 1201 GGCCGCCGCT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt