Construct: ORF TRCN0000469850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013717.1_s317c1
- Derived from:
- ccsbBroadEn_12991
- DNA Barcode:
- CGTCTCGAGCCATGAACCATGAGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM44 (93109)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469850
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_005269371.4 | 38.9% | 38.9% | 1_804del |
| 2 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_138399.5 | 38.7% | 38.7% | 1_804del;1174_1175insAGC |
| 3 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_017007517.1 | 38.6% | 38.6% | 1_813del |
| 4 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513322.2 | 37.3% | 37.3% | 1_804del;911_967del |
| 5 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513321.2 | 37% | 37% | 1_813del;920_976del |
| 6 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513319.2 | 36.5% | 36.5% | 1_834del;941_997del |
| 7 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001011655.3 | 36.4% | 36.4% | 1_804del;1174_1175ins33 |
| 8 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_017007518.1 | 36.1% | 36.1% | 1_813del;1183_1184ins33 |
| 9 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513320.2 | 33.8% | 33.8% | 1_945del;1052_1108del |
| 10 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001166305.2 | 32.9% | 32.9% | 1_945del;1315_1316ins33 |
| 11 | human | 93109 | TMEM44 | transmembrane protein 44 | XM_011513318.2 | 32.7% | 32.7% | 1_954del;1324_1325ins33 |
| 12 | human | 93109 | TMEM44 | transmembrane protein 44 | NM_001166306.2 | 29.1% | 18.2% | (many diffs) |
| 13 | mouse | 224090 | Tmem44 | transmembrane protein 44 | XM_006522063.3 | 22.2% | 21.1% | (many diffs) |
| 14 | mouse | 224090 | Tmem44 | transmembrane protein 44 | XM_006522064.3 | 22% | 20.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 579
- ORF length:
- 513
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa gagcaagatg agacaggcct taggatttgc caaggaagcc agagagagcc 121 ctgacaccca agcccttttg acctgtgcag agaaagagga agaaaaccag gagaatttgg 181 attgggtgcc tctcaccaca ctgtcacact gcaagtcact gaggacaatg acagcaatca 241 gtcgctacat ggagctgacc atcgagcctg tgcagcaggc aggctgcagt gccaccaggc 301 tgccaggtga cgggcagacg agcgccggag atgcgtccct gcaggacccc ccgtcgtacc 361 ctcccgttca ggtcatccgg gcccgggtgt cttccggcag ctcctctgag gtctcctcca 421 tcaactccga cctggagcag aagtattggg aggccctaaa ctcggagcag tgggaccctg 481 aagatgtgaa cctcgaaggc agcaaagaaa atgtggagct actgggaTCC CAGGTGCACC 541 AGGACTCTGT GAGGACAGCA CACCTGAGTG ATGATGATTA CCCAACTTTC TTGTACAAAG 601 TGGTTGATAT CGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT 661 GAACTAGTCC GTAACTTGAA AGTATTTCGA TTTCTTGGCC TTATATATCT TGTGGAAAGG 721 ACGACGTCTC GAGCCATGAA CCATGAGGAC GCGTTAAGTC gacaatcaac ctctggatta 781 caaaatttgt gaaagatt