Construct: ORF TRCN0000469861
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003367.3_s317c1
- Derived from:
- ccsbBroadEn_15057
- DNA Barcode:
- GCCGCTTCATGTGAACTCCGGGCT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- AK3 (50808)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469861
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 50808 | AK3 | adenylate kinase 3 | NM_016282.4 | 100% | 100% | |
| 2 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199852.2 | 82.3% | 82.3% | 150_151ins120 |
| 3 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199853.1 | 69.1% | 69.1% | 0_1ins210 |
| 4 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199855.1 | 69.1% | 69.1% | 0_1ins210 |
| 5 | human | 50808 | AK3 | adenylate kinase 3 | NM_001199856.1 | 69.1% | 69.1% | 0_1ins210 |
| 6 | mouse | 56248 | Ak3 | adenylate kinase 3 | NM_021299.1 | 88.8% | 92.5% | (many diffs) |
| 7 | mouse | 56248 | Ak3 | adenylate kinase 3 | XM_006527222.3 | 71.7% | 64.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 747
- ORF length:
- 681
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ggcgtccgcg cggctgctgc gagcggtgat catgggggcc ccgggctcgg 121 gcaagggcac cgtgtcgtcg cgcatcacta cacacttcga gctgaagcac ctctccagcg 181 gggacctgct ccgggacaac atgctgcggg gcacagaaat tggcgtgtta gccaaggctt 241 tcattgacca agggaaactc atcccagatg atgtcatgac tcggctggcc cttcatgagc 301 tgaaaaatct cacccagtat agctggctgt tGGATGGTTT TCCAAGGACA CTTCCACAGG 361 CAGAAGCCCT AGATAGAGCT TATCAGATCG ACACAGTGAT TAACCTGAAT GTGCCCTTTG 421 AGGTCATTAA ACAACGCCTT ACTGCTCGCT GGATTCATCC CGCCAGTGGC CGAGTCTATA 481 ACATTGAATT CAACCCTCCC AAAACTGTGG GCATTGATGA CCTGACTGGG GAGCCTCTCA 541 TTCAGCGTGA GGATGATAAA CCAGAGACGG TTATCAAGAG ACTAAAGGCT TATGAAGACC 601 AAACAAAGCC AGTCCTGGAA TATTACCAGA AAAAAGGGGT GCTGGAAACA TTCTCCGGAA 661 CAGAAACCAA CAAGATTTGG CCCTATGTAT ATGCTTTCCT ACAAACTAAA GTTCCACAAA 721 GAAGCCAGAA AGCTTCAGTT ACTCCATGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 781 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 841 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGCCGCTTC 901 ATGTGAACTC CGGGCTACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 961 aagatt