Construct: ORF TRCN0000469862
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018946.2_s317c1
- Derived from:
- ccsbBroadEn_14661
- DNA Barcode:
- TTCATGTATTTTACGTTTCATCGC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- GSK3A (2931)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469862
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | NM_019884.3 | 99.7% | 49.8% | 707_708insC;727_728insG;759delC |
| 2 | human | 2931 | GSK3A | glycogen synthase kinase 3 ... | XR_001753673.1 | 54.8% | (many diffs) | |
| 3 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | NM_001031667.1 | 88.8% | 47.9% | (many diffs) |
| 4 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540258.1 | 83.5% | 52.3% | (many diffs) |
| 5 | mouse | 606496 | Gsk3a | glycogen synthase kinase 3 ... | XM_006540259.3 | 77.1% | 35.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 831
- ORF length:
- 762
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gagcggcggc gggccttcgg gaggcggccc tgggggctcg ggcagggcgc 121 ggactagctc gttcgcggag cccggcggcg gaggcggagg aggcggcggc ggccccggag 181 gctcggcctc cggcccaggc ggcaccggcg gcggaaaggc atctgtcggg gccatgggtg 241 ggggcgtcgg ggcctcgagc tccgggggtg gacccggcgg cagcggcgga ggaggcagcg 301 gaggccccgg cgcaggcact agcttcccgc cgcccggggt gaagctgggc cgtgacagcg 361 ggaaggtgac cacagtcgta gccactctag gccaaggccc agagcgctcc caagaagtgg 421 cttacacgga catcaaagtg attggcaatg gctcatttgg ggtcgtgtac caggcacggc 481 tggcagagac cagggaacta gtcgccatca agaaggttct ccaggacaag aggttcaaga 541 accgagagct gcagatcatg cgtaagctgg accactgcaa tattgtgagg ctgagatact 601 ttttctactc cagtggcgag aagaaagacg agctttacct aaatctggtg ctggaatatg 661 tgcccgagac agtgtaccgg gtggcccgcc acttcaccaa ggccaagttg accatcccta 721 tcctctatgt caaggtgtac atgtaccagc tcttccgcag cttggcctac atccacctcc 781 cagggcgtgt gtcaccggcg acatcaagcc ccagaacctg ctggtggacc tgacactgct 841 gtcctcaagc tctgcgattt tggcagtgca aagcagttgg tccgagggga gcccaatgtc 901 tcctacatct gttctcgcta ctaccgggcc ccagagctca tctttggagc cactgattac 961 acctcatcca tcgatgtttg gtcagctggc tgtgtactgg cagagctcct cttgggccag 1021 cccatcttcc ctggggacag tggggtggac cagctggtgg agatcatcaa ggtgctggga 1081 acaccaaccc gggaacaaat ccgagagatg aaccccaact acacggagtt caagttccct 1141 cagattaaag ctcacccctg gacaaaggtg ttcaaaTCTC GAACGCCGCC AGAGGCCATC 1201 GCGCTCTGCT CTAGCCTGCT GGAGTACACC CCATCCTCAA GGCTCTCCCC ACTAGAGGCC 1261 TGTGCGCACA GCTTCTTTGA TGAACTGCGA TGTCTGGGAA CCCAGCTGCC TAACAACCGC 1321 CCACTTCCCC CTCTCTTCAA CTTCAGTGCT GGTGAACTCT CCATCCAACC GTCTCTCAAC 1381 GCCATTCTCA TCCCTCCTCA CTTGAGGTCC CCAGCGGGCA CTACCACCCT CACCCCGTCC 1441 TCACAAGCTT TAACTGAGAC TCCGACCAGC TCAGACTGGC AGTCGACCGA TGCCACACCT 1501 ACCCTCACTA ACTCCTCCTT GCCAACTTTC TTGTACAAAG TGGTTGATAT CGGTAAGCCT 1561 ATCCCTAACC CTCTCCTCGG TCTCGATTCT ACGTAGTAAT GAACTAGTCC GTAACTTGAA 1621 AGTATTTCGA TTTCTTGGCT TTATATATCT TGTGGAAAGG ACGATTCATG TATTTTACGT 1681 TTCATCGCAC GCGTTAAGTC gacaatcaac ctctggatta caaaatttgt gaaagatt