Construct: ORF TRCN0000469864

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018824.1_s317c1
Derived from:
ccsbBroadEn_15291
DNA Barcode:
TTGTGTCTGGGCTCAGCACACATC
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PRPS1L1 (221823)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469864
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 221823 PRPS1L1 phosphoribosyl pyrophosphat... NM_175886.3 99.8% 1.8% 187A>N
2 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_002764.4 92.1% 1.5% (many diffs)
3 human 5631 PRPS1 phosphoribosyl pyrophosphat... NM_001204402.1 31.9% 4.3% (many diffs)
4 mouse 328099 Prps1l3 phosphoribosyl pyrophosphat... NM_001037746.3 89.9% 1.5% (many diffs)
5 mouse 19139 Prps1 phosphoribosyl pyrophosphat... NM_021463.4 89.6% 1.5% (many diffs)
6 mouse 75456 Prps1l1 phosphoribosyl pyrophosphat... NM_029294.2 87.3% 1.2% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
202
ORF end:
268
ORF length:
66
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcaccac gccgaatatc aaaatcttca gcggcagctc ccaccaggac ttatcccaga
121aaattgctga ccgcctgggc ctggagctag gcaaggtggt gactaagaaa ttcagcaacc
181aggagacctg cgtggaaatt gatgagagtg tgcgtggaga ggatgtctac atcgttcaga
241gtggttgtgg cgaantcaac gacagtctaa tggagctttt gatcatgatt aatgcctgca
301agattgcttc agctagccga gttactgcag tcatcccatg cttcccttat gcccgacagg
361ataagaagga taagagccgg tccccaatct ctgccaagct tgttgcaaat atgctctcta
421tagcaggtgc ggatcatatc atcaccatgg acctacatgc ttctcaaatt cagggctttt
481ttgatatccc agtagacaac ttgtatgcag agccaactgt cctgaagtgg ataagggaga
541atatccctga gtggaagaac tgcattattg tctcgccaga tgctggtgga gctaaaagag
601tgacctccat tgcagaccag ttgaatgtgg actttgcttt gattcataaa gaacggaaga
661aggCCAATGA AGTGGACTGC ATAGTGCTAG TGGGAGATGT GAATGATCGT GTGGCTATCC
721TTGTAGATGA CATGGCAGAC ACTTGTGTTA CAATCTGCCT CGCAGCTGAC AAACTTCTCT
781CAGCTGGAGC AACCAGAGTT TATGCTATCT TGACTCATGG AATCTTTTCT GGCCCAGCCA
841TTTCTCGCAT CAACACTGCA TGCTTTGAAG CAGTGGTAGT CACCAATACC ATACCTCAAG
901ATGAGAAGAT GAAGCATTGC TCCAAAATAC GAGTAATTGA CATCTCCATG ATCCTTGCAG
961AAGCCATAAG GAGAACTCAT AATGGGGAAT CTGTTTCCTA CCTGTTCAGC CATGTTCCTT
1021TATTGCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC
1081TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT
1141GGCTTTATAT ATCTTGTGGA AAGGACGATT GTGTCTGGGC TCAGCACACA TCACGCGTTA
1201AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt

Download FASTA (ORF) (Full)