Construct: ORF TRCN0000469867
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001467.1_s317c1
- Derived from:
- ccsbBroadEn_01593
- DNA Barcode:
- GCGAAGCAAAGATTAAGTCCGACC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SRP14 (6727)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469867
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6727 | SRP14 | signal recognition particle 14 | NM_003134.6 | 100% | 100% | |
| 2 | human | 6727 | SRP14 | signal recognition particle 14 | NM_001309434.1 | 50% | 44.9% | 0_1ins184;26_65del |
| 3 | mouse | 20813 | Srp14 | signal recognition particle 14 | NM_009273.4 | 70.5% | 72% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 474
- ORF length:
- 408
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gttgttggag agcgagcagt tcctgacgga gctgaccaga cttttccaga 121 agtgccggac gtcgggcagc gtctatatca ccttgaagaa gtatgacggt cgaaccaaac 181 ccattccaaa gaagggtact gtggagggct ttgagcccgc agacaacaag tgtctgttaa 241 gagctaccga tgggaagaag aagatcagca ctgtggtgag ctccaaggaa gtgaataagt 301 ttcagatggc ttattcaaac ctccttagag cTAACATGGA TGGGCTGAAG AAGAGAGACA 361 AAAAGAACAA AACTAAGAAG ACCAAAGCAG CAGCAGCAGC AGCAGCAGCA GCACCTGCCG 421 CAGCAGCAAC AGCACCAACA ACAGCAGCAA CAACAGCAGC AACAGCAGCA CAGTACCCAA 481 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 541 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 601 TATCTTGTGG AAAGGACGAG CGAAGCAAAG ATTAAGTCCG ACCACGCGTT AAGTCgacaa 661 tcaacctctg gattacaaaa tttgtgaaag att