Construct: ORF TRCN0000469868
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013237.1_s317c1
- Derived from:
- ccsbBroadEn_13486
- DNA Barcode:
- GCACTGACACATTAACGCTCCAGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NALCN (259232)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469868
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350750.2 | 12.6% | 12.6% | (many diffs) |
| 2 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350751.2 | 12.6% | 12.6% | (many diffs) |
| 3 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_011521069.2 | 12.5% | 12.5% | (many diffs) |
| 4 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350749.2 | 12.4% | 12.4% | (many diffs) |
| 5 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_052867.4 | 12.4% | 12.4% | (many diffs) |
| 6 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_011521067.2 | 12.3% | 12.2% | (many diffs) |
| 7 | human | 259232 | NALCN | sodium leak channel, non-se... | NM_001350748.2 | 12.2% | 12.2% | (many diffs) |
| 8 | human | 259232 | NALCN | sodium leak channel, non-se... | XM_024449336.1 | 12.1% | 12% | (many diffs) |
| 9 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316066.2 | 16.2% | 17.8% | (many diffs) |
| 10 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | NM_177393.4 | 11.3% | 12.4% | (many diffs) |
| 11 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_030247866.1 | 11.2% | 12.3% | (many diffs) |
| 12 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316064.2 | 11.2% | 12.3% | (many diffs) |
| 13 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316063.2 | 11.1% | 12.2% | (many diffs) |
| 14 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_011245094.3 | 11.1% | 12.1% | (many diffs) |
| 15 | mouse | 338370 | Nalcn | sodium leak channel, non-se... | XM_017316062.2 | 11% | 12% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 720
- ORF length:
- 654
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgct caaaaggaag cagagttcca gggtggaagc ccagccagtc actgactttg 121 gtcctgatga gtctctgtcg gataatgctg acatcctctg gattaacaaa ccatgggttc 181 actctttgct gcgcatctgt gccatcatca gcgtcatttc tgtttgtatg aatacgccaa 241 tgaccttcga gcactatcct ccacttcagt atgtgacctt cactttGGAT ACATTATTGA 301 TGTTTCTCTA CACGGCAGAG ATGATAGCAA AAATGCACAT CCGGGGCATT GTCAAGGGGG 361 ATAGTTCCTA TGTGAAAGAT CGCTGGTGTG TTTTTGATGG ATTTATGGTC TTTTGCCTTT 421 GGGTTTCTTT GGTGCTACAG GTGTTTGAAA TTGCTGATAT AGTTGATCAG ATGTCACCTT 481 GGGGCATGTT GCGGATTCCA CGGCCACTGA TTATGATCCG AGCATTCCGG ATTTATTTCC 541 GATTTGAACT GCCAAGGACC AGAATTACAA ATATTTTAAA GCGATCGGGA GAACAAATAT 601 GGAGTGTTTC CATTTTTCTA CTTTTCTTTC TACTTCTTTA TGGAATTTTA GGAGTTCAGA 661 TGTTTGGAAC ATTTACTTAT CACTGTGTTG TAAATGACAC AAAGCCAGGA CTCTCACTCT 721 GCCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 781 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 841 TTTATATATC TTGTGGAAAG GACGAGCACT GACACATTAA CGCTCCAGCA CGCGTTAAGT 901 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt