Transcript: Human XM_024449336.1

PREDICTED: Homo sapiens sodium leak channel, non-selective (NALCN), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NALCN (259232)
Length:
6954
CDS:
73..5433

Additional Resources:

NCBI RefSeq record:
XM_024449336.1
NBCI Gene record:
NALCN (259232)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024449336.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000044433 CGGTGGAGTAATGGACATATT pLKO.1 3075 CDS 100% 13.200 18.480 N NALCN n/a
2 TRCN0000420381 TTCGAACTACTACTCGTAATT pLKO_005 1486 CDS 100% 13.200 18.480 N NALCN n/a
3 TRCN0000044435 GCACGCTTCAACGCATCTAAA pLKO.1 2782 CDS 100% 13.200 10.560 N NALCN n/a
4 TRCN0000413962 GCCGATCCATGGAATCTATAT pLKO_005 3603 CDS 100% 13.200 10.560 N NALCN n/a
5 TRCN0000174609 GAGAACAAATATGGAGTGTTT pLKO.1 653 CDS 100% 4.950 3.465 N Nalcn n/a
6 TRCN0000044436 CCCAACATTATTAGAAGGGAA pLKO.1 3361 CDS 100% 2.640 1.848 N NALCN n/a
7 TRCN0000044434 CCAGGGAATGTAACCTGGAAT pLKO.1 769 CDS 100% 0.495 0.347 N NALCN n/a
8 TRCN0000044437 GCCATCTATTTCATTCTCTAT pLKO.1 1855 CDS 100% 4.950 2.970 N NALCN n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024449336.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13485 pDONR223 100% 36.1% 32.3% None (many diffs) n/a
2 ccsbBroad304_13485 pLX_304 0% 36.1% 32.3% V5 (many diffs) n/a
3 TRCN0000476819 AGTCCACTTCATTGGAAGCTGTTC pLX_317 18.1% 36.1% 32.3% V5 (many diffs) n/a
4 ccsbBroadEn_13486 pDONR223 100% 12.1% 12% None (many diffs) n/a
5 ccsbBroad304_13486 pLX_304 0% 12.1% 12% V5 (many diffs) n/a
6 TRCN0000469868 GCACTGACACATTAACGCTCCAGC pLX_317 65.8% 12.1% 12% V5 (many diffs) n/a
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