Construct: ORF TRCN0000469876
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009189.1_s317c1
- Derived from:
- ccsbBroadEn_02676
- DNA Barcode:
- TTCTACGGCAAACCCGTACGCGAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PHB2 (11331)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469876
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 11331 | PHB2 | prohibitin 2 | NM_001144831.2 | 100% | 100% | |
| 2 | human | 11331 | PHB2 | prohibitin 2 | NM_001267700.1 | 87.2% | 87.2% | 606_607ins114 |
| 3 | human | 11331 | PHB2 | prohibitin 2 | XR_242980.3 | 58.5% | (many diffs) | |
| 4 | mouse | 12034 | Phb2 | prohibitin 2 | NM_007531.2 | 91.3% | 100% | (many diffs) |
| 5 | mouse | 12034 | Phb2 | prohibitin 2 | XR_001785095.1 | 38.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 963
- ORF length:
- 897
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ccagaacttg aaggacttgg cgggacggct gcccgccggg ccccggggca 121 tgggcacggc cctgaagctg ttgctggggg ccggcgccgt ggcctacggt gtgcgcgaat 181 ctgtgttcac cgtggaaggc gggcacagag ccatcttctt caatcggatc ggtggagtgc 241 agcaggacac tatcctggcc gagggccttc acttcaggat cccttggttc cagtacccca 301 ttatctatga cattcgggcc agacctcgaa aaatctcctc ccctacaggc tccaaagacc 361 tacagatggt gaatatctcc ctgcgagtgt tgtctcgacc caatgctcag gagcttccta 421 gcatgtacca gcgcctaggg ctggactacg aggaacgagt gttgccgtcc attgtcaacg 481 aggtgctcaa gagtgtggtg gccaagttca atgcctcaca gctgatcacc cagcgggccc 541 aggtatccct gttgatccgc cgggagctga cagagagggc caaggacttc agccTCATCC 601 TGGATGATGT GGCCATCACA GAGCTGAGCT TTAGCCGAGA GTACACAGCT GCTGTAGAAG 661 CCAAACAAGT GGCCCAGCAG GAGGCCCAGC GGGCCCAATT CTTGGTAGAA AAAGCAAAGC 721 AGGAACAGCG GCAGAAAATT GTGCAGGCCG AGGGTGAGGC CGAGGCTGCC AAGATGCTTG 781 GAGAAGCACT GAGCAAGAAC CCTGGCTACA TCAAACTTCG CAAGATTCGA GCAGCCCAGA 841 ATATCTCCAA GACGATCGCC ACATCACAGA ATCGTATCTA TCTCACAGCT GACAACCTTG 901 TGCTGAACCT ACAGGATGAA AGTTTCACCA GGGGAAGTGA CAGCCTCATC AAGGGTAAGA 961 AATGCCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 1021 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 1081 GGCTTTATAT ATCTTGTGGA AAGGACGATT CTACGGCAAA CCCGTACGCG ACACGCGTTA 1141 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt