Construct: ORF TRCN0000469879
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015254.1_s317c1
- Derived from:
- ccsbBroadEn_13377
- DNA Barcode:
- GTGTATAACTTGATTCATGGCTCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NSMCE1 (197370)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469879
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | NM_145080.4 | 96.1% | 95.8% | 1_30del;140A>G |
| 2 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XM_006721023.4 | 96.1% | 95.8% | 1_30del;140A>G |
| 3 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XM_011545762.1 | 66.4% | 66.4% | 0_1ins258 |
| 4 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XM_017023040.1 | 66.4% | 66.4% | 0_1ins258 |
| 5 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XM_017023041.1 | 66.4% | 66.4% | 0_1ins258 |
| 6 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XM_017023042.1 | 66.4% | 66.4% | 0_1ins258 |
| 7 | human | 197370 | NSMCE1 | NSE1 homolog, SMC5-SMC6 com... | XR_002957792.1 | 40% | (many diffs) | |
| 8 | mouse | 67711 | Nsmce1 | NSE1 homolog, SMC5-SMC6 com... | XM_006508135.3 | 82.9% | 86% | (many diffs) |
| 9 | mouse | 67711 | Nsmce1 | NSE1 homolog, SMC5-SMC6 com... | XM_006508136.2 | 82.9% | 86% | (many diffs) |
| 10 | mouse | 67711 | Nsmce1 | NSE1 homolog, SMC5-SMC6 com... | XM_017312269.1 | 82.9% | 86% | (many diffs) |
| 11 | mouse | 67711 | Nsmce1 | NSE1 homolog, SMC5-SMC6 com... | NM_026330.3 | 78.8% | 81.7% | (many diffs) |
| 12 | mouse | 67711 | Nsmce1 | NSE1 homolog, SMC5-SMC6 com... | XM_006508134.3 | 74.6% | 77.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 834
- ORF length:
- 768
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgac tgatgtccac cggcgcttcc tccagttgct gatgacccat ggcgtgctag 121 aggaatggga cgtgaagcgc ttgcagacgc actgctacaa ggtccatgac cgcagtgcca 181 ccgtagataa gttggaggac ttcatcaaca acattaacag tgtcttggag tccttgtata 241 ttgagataaa gagaggagtc acggaagatg atgggagacc catttatgcg ttggtgaatc 301 ttgctacaac ttcaatttcc aaaatggcta cggattttgc agagaatgaa ctggatttgt 361 ttagaaaggc tctggaactg attattgact cagaaaccgg cttTGCGTCT TCCACAAACA 421 TATTGAACCT GGTTGATCAA CTTAAAGGCA AGAAGATGAG GAAGAAGGAA GCGGAGCAGG 481 TGCTGCAGAA GTTTGTTCAA AACAAGTGGC TGATTGAGAA GGAAGGGGAG TTCACCCTGC 541 ACGGCCGGGC CATCCTGGAG ATGGAGCAAT ACATCCGGGA GACGTACCCC GACGCGGTGA 601 AGATCTGCAA TATCTGTCAC AGCCTCCTCA TCCAGGGTCA AAGCTGCGAA ACCTGTGGGA 661 TCAGGATGCA CTTACCCTGC GTGGCCAAGT ACTTCCAGTC GAATGCTGAA CCGCGCTGCC 721 CCCACTGCAA CGACTACTGG CCCCACGAGA TCCCAAAAGT CTTCGACCCT GAGAAGGAGA 781 GGGAGTCTGG TGTCTTGAAA TCGAACAAAA AGTCCCTGCG GTCCAGGCAG CATTACCCAA 841 CTTTCTTGTA CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG 901 ATTCTACGTA GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA 961 TATCTTGTGG AAAGGACGAG TGTATAACTT GATTCATGGC TCCACGCGTT AAGTCgacaa 1021 tcaacctctg gattacaaaa tttgtgaaag att