Construct: ORF TRCN0000469880

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008244.1_s317c1
Derived from:
ccsbBroadEn_08789
DNA Barcode:
TCAGTTATAAATAGTTGCAGCACG
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
AFAP1 (60312)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469880
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 60312 AFAP1 actin filament associated p... NM_001371090.1 99.8% 99.7% 297G>A;1208C>G;2176T>A
2 human 60312 AFAP1 actin filament associated p... NM_001371091.1 99.8% 99.7% 297G>A;1208C>G;2176T>A
3 human 60312 AFAP1 actin filament associated p... NM_198595.2 99.8% 99.7% 297G>A;1208C>G;2176T>A
4 human 60312 AFAP1 actin filament associated p... XM_017008535.1 97.4% 97.3% (many diffs)
5 human 60312 AFAP1 actin filament associated p... XM_006713909.3 97% 96.9% (many diffs)
6 human 60312 AFAP1 actin filament associated p... NM_001134647.1 89.5% 89.4% (many diffs)
7 human 60312 AFAP1 actin filament associated p... XM_011513544.3 89.5% 89.4% (many diffs)
8 human 60312 AFAP1 actin filament associated p... XM_017008536.1 69.9% 71.3% (many diffs)
9 mouse 70292 Afap1 actin filament associated p... NM_027373.2 84.3% 91.3% (many diffs)
10 mouse 70292 Afap1 actin filament associated p... XM_006504098.2 75.3% 81.4% (many diffs)
11 mouse 70292 Afap1 actin filament associated p... XM_006504100.3 63.9% 68.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2256
ORF length:
2190
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgga agagttaata gttgaacttc gtctctttct tgaactcctg gaccatgaat
121atctaacctc aactgtcagg gagaaaaagg cagtgataac caacattctg ctaagaatac
181agtcatccaa aggttttgat gtgaaggacc atgctcagaa gcaggagacc gctaacagcc
241tgccagcccc tcctcagatg cccctgccgg agatccctca gccctggctg cctcctgaca
301gtgggcctcc accattgcca acatcctccc tcccagaagg ttattatgag gaagctgtgc
361cactgagccc cggaaaagct ccggaataca tcacatcaaa ttatgattcc gatgcgatga
421gcagctctta tgagtcgtat gatgaagagg aggaggatgg gaaggggaag aaaacccggc
481accagtggcc ctccgaggag gcctccatgg acctggtcaa ggacgccaaa atctgcgcct
541tcctgctgcg gaagaagcgg ttcggccagt ggaccaagtt gctctgcgtc atcaaagaca
601ccaaactgct gtgctataaa agttccaagg accagcagcc tcagatggaa ctgccactcc
661aaggctgtaa cattacgtac atcccgaaag acagcaaaaa gaagaagcac gagctgaaga
721ttactcagca gggcacggac ccgcttgttc tcgccgtcca gagcaaggaa caggccgagc
781agtggctgaa ggtgatcaaa gaagcctaca gtggttgtag tggccccgtg gattcagagt
841gtcctcctcc accaagctcc ccggtgcaca aggcagaact ggagaagaaa ctgtcttcag
901agagacccag ctcagatggg gagggtgttg tggaaaatgg aattaccaca tgtaatggaa
961aggagcaagt gaagaggaag aaaagttcca aatcagaggc caagggcact gtgtcgaaag
1021tcactgggaa aaaaatcacc aagatcatca gtctgggaaa gaaaaagccg tccacagacg
1081agcagacctc ctcagctgag gaagatgttc ccacctgcgg ctatctgaac gtgctctcca
1141acagccgctg gcgagagcgc tggtgccgag tgaaagataa caagctcatt ttccacaagg
1201acaggaccga cctgaagacc catattgtgt ctattccgct ccgtggctgc gaggtgatcc
1261cgggtttgga ttgtaaacat cctctgacgt tccggctgct gcgcaacggc caggaggttg
1321cagtattgga ggcatcttct tctgaagaca tgggcaggtg gattgggatt ttactcgcag
1381agacgggatc gtccacagac ccggaggctc tgcactatga ctacattgat gtggagatgt
1441ctgcaagtgt cattcagaca gccaaacaga ccttctgttt catgaacagg cgtgttatat
1501ctgctaaccc atatctaggg ggcacctcca acggctatgc ccaccccagc gggacggcac
1561ttcattatga cgatgtcccg tgcatcaacg gctcgctcaa gggtaaaaag ccccccgtgg
1621cgtctaatgg ggtcacagga aaagggaaga ctctgagcag tcagccaaag aaagcggatc
1681ccgcggctgt tgtgaaaagg acgggttcga atgctgccca gtacaagtat ggcaagaacc
1741gggtagaagc agatgccaag cggctacaga ccaaagagga ggagctgctg aagaggaaag
1801aggccctgcg gaataggctg gcccagcTCC GCAAGGAAAG AAAAGACCTT CGAGCGGCTA
1861TTGAAGTGAA CGCCGGCAGG AAGCCGCAGG CGATCCTGGA GGAGAAGCTG AAGCAGCTGG
1921AGGAGGAGTG CCGGCAGAAG GAGGCGGAGC GTGTCAGCCT GGAGCTGGAG CTGACGGAGG
1981TCAAGGAGAG CCTGAAGAAA GCGCTGGCGG GCGGAGTCAC CCTGGGGCTG GCCATCGAGC
2041CCAAGTCAGG GACATCGAGT CCACAGTCTC CAGTGTTCCG GCACCGGACC CTGGAAAACT
2101CGCCCATCTC CAGCTGTGAC ACCAGTGACA CCGAGGGCCC CGTGCCGGTG AACAGCGCGG
2161CCGTCTTGAA GAAGAGCCAG GCTGCCCCGG GCAGCTCCCC CTGCCGAGGG CATGTGCTGC
2221GGAAGGCCAA GGAATGGGAA ATGAAGAACG GGACCTACCC AACTTTCTTG TACAAAGTGG
2281TTGATATCGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA
2341CTAGCCCGTA ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG
2401ATCAGTTATA AATAGTTGCA GCACGACGCG TTAAGTCgac aatcaacctc tggattacaa
2461aatttgtgaa agatt

Download FASTA (ORF) (Full)