Construct: ORF TRCN0000469885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015092.1_s317c1
- Derived from:
- ccsbBroadEn_05385
- DNA Barcode:
- TGTCGTATCTACACTTCCGCCGTG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RSPO1 (284654)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469885
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 284654 | RSPO1 | R-spondin 1 | NM_001038633.4 | 100% | 100% | |
| 2 | human | 284654 | RSPO1 | R-spondin 1 | NM_001242908.2 | 100% | 100% | |
| 3 | human | 284654 | RSPO1 | R-spondin 1 | XM_006710583.4 | 100% | 100% | |
| 4 | human | 284654 | RSPO1 | R-spondin 1 | NM_001242909.2 | 88.2% | 88.2% | (many diffs) |
| 5 | human | 284654 | RSPO1 | R-spondin 1 | NM_001242910.2 | 76% | 76% | 435_436ins189 |
| 6 | mouse | 192199 | Rspo1 | R-spondin 1 | NM_138683.2 | 85.8% | 87.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 858
- ORF length:
- 789
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat gcggcttggg ctgtgtgtgg tggccctggt tctgagctgg acgcacctca 121 ccatcagcag ccgggggatc aaggggaaaa ggcagaggcg gatcagtgcc gaggggagcc 181 aggcctgtgc caaaggctgt gagctctgct ctgaagtcaa cggctgcctc aagtgctcac 241 ccaagctgtt catcctgctg gagaggaacg acatccgcca ggtgggcgtc tgcttgccgt 301 cctgcccacc tggatacttc gacgcccgca accccgacat gaacaagtgc atcaaatgca 361 agatcgagca ctgtgaggcc tgcttcagcc ataacttctg caccaagtgt aaggagggct 421 tgtacctgca caagggccgc tgctatccag cttgtcccga gggctccTCA GCTGCCAATG 481 GCACCATGGA GTGCAGTAGT CCTGCGCAAT GTGAAATGAG CGAGTGGTCT CCGTGGGGGC 541 CCTGCTCCAA GAAGCAGCAG CTCTGTGGTT TCCGGAGGGG CTCCGAGGAG CGGACACGCA 601 GGGTGCTACA TGCCCCTGTG GGGGACCATG CTGCCTGCTC TGACACCAAG GAGACCCGGA 661 GGTGCACAGT GAGGAGAGTG CCGTGTCCTG AGGGGCAGAA GAGGAGGAAG GGAGGCCAGG 721 GCCGGCGGGA GAATGCCAAC AGGAACCTGG CCAGGAAGGA GAGCAAGGAG GCGGGTGCTG 781 GCTCTCGAAG ACGCAAGGGG CAGCAACAGC AGCAGCAGCA AGGGACAGTG GGGCCACTCA 841 CATCTGCAGG GCCTGCCTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 901 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 961 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATGTCGTA TCTACACTTC 1021 CGCCGTGACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt