Transcript: Mouse NM_138683.2

Mus musculus R-spondin 1 (Rspo1), mRNA.

Source:
NCBI, updated 2017-06-26
Taxon:
Mus musculus (mouse)
Gene:
Rspo1 (192199)
Length:
1834
CDS:
483..1280

Additional Resources:

NCBI RefSeq record:
NM_138683.2
NBCI Gene record:
Rspo1 (192199)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_138683.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000127457 AGAAGTCAACGGTTGCCTCAA pLKO.1 626 CDS 100% 4.050 5.670 N Rspo1 n/a
2 TRCN0000127456 CACTCACATCAGTAGGACCTA pLKO.1 1246 CDS 100% 2.640 3.696 N Rspo1 n/a
3 TRCN0000127458 TGGATACTTTGATGCCCGCAA pLKO.1 725 CDS 100% 2.160 1.728 N Rspo1 n/a
4 TRCN0000127455 GACATGAACAAATGCATCAAA pLKO.1 750 CDS 100% 5.625 3.375 N Rspo1 n/a
5 TRCN0000127454 CCCATCCAACTATCCATCCAT pLKO.1 1384 3UTR 100% 3.000 1.800 N Rspo1 n/a
6 TRCN0000166364 CACACACACACACACACACAA pLKO.1 1444 3UTR 100% 4.950 2.475 Y KAAG1 n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_138683.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05385 pDONR223 100% 85.8% 87.9% None (many diffs) n/a
2 ccsbBroad304_05385 pLX_304 0% 85.8% 87.9% V5 (many diffs) n/a
3 TRCN0000469885 TGTCGTATCTACACTTCCGCCGTG pLX_317 49.3% 85.8% 87.9% V5 (many diffs) n/a
Download CSV