Construct: ORF TRCN0000469903
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009187.1_s317c1
- Derived from:
- ccsbBroadEn_06070
- DNA Barcode:
- CTACTGGGGTTACGGTAGGATCGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CYC1 (1537)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469903
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 1537 | CYC1 | cytochrome c1 | NM_001916.5 | 99.7% | 99.6% | 99T>G;226A>G |
| 2 | human | 1537 | CYC1 | cytochrome c1 | XM_024447072.1 | 81.7% | 81.5% | 0_1ins177;49A>G |
| 3 | human | 1537 | CYC1 | cytochrome c1 | XM_017013102.1 | 63.8% | 56% | (many diffs) |
| 4 | mouse | 66445 | Cyc1 | cytochrome c-1 | NM_025567.2 | 87.6% | 90.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1041
- ORF length:
- 975
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcagctgcg gcttcgcttc gcggggtagt gttgggcccg cggggcgcgg 121 ggctcccggg cgcgcgtgcc cggggtctgc tgtgcagcgc gcggcccggg cagctcccgc 181 tacggacacc tcaggcagtg gccttgtcgt cgaagtctgg cctttcccga ggccggaaag 241 tgatgctgtc agcgctgggc atgctggcgg cagggggtgc ggggctggcc gtggctctgc 301 attcggctgt gagtgccagt gacctggagc tgcacccccc cagctatccg tggtctcacc 361 gtggcctcct ctcttccttg gaccacacca gcatccggag gggtttccag gtatataagc 421 aggtgtgcgc ctcctgccac agcatggact tcgtggccta ccgccacctg gtgggcgtgt 481 gctacacgga ggatgaagct aaggagctgg ctgcggaggt ggaggttcaa gacggcccca 541 atgaagatgg ggagatgttc atgcggccag ggaagctgtt cgactatttc ccaaaaccat 601 accccaacag tgaggctgct cgagctgcca acaacggagc attgccccct gacctcagct 661 acatcgtgcg agctaggcat ggtggtgagg actacgtcTT CTCCCTGCTC ACGGGCTACT 721 GCGAGCCACC CACCGGGGTG TCACTGCGGG AAGGTCTCTA CTTCAACCCC TACTTTCCTG 781 GCCAGGCCAT TGCCATGGCC CCTCCCATCT ACACAGATGT CTTAGAGTTT GACGATGGCA 841 CCCCAGCTAC CATGTCCCAG ATAGCCAAGG ATGTGTGCAC CTTCCTGCGC TGGGCATCTG 901 AGCCAGAGCA CGACCATCGA AAACGCATGG GGCTCAAGAT GTTGATGATG ATGGCTCTGC 961 TGGTGCCCCT GGTCTACACC ATAAAGCGGC ACAAGTGGTC AGTCCTGAAG AGTCGGAAGC 1021 TGGCATATCG GCCGCCCAAG TGCCCAACTT TCTTGTACAA AGTGGTTGAT ATCGGTAAGC 1081 CTATCCCTAA CCCTCTCCTC GGTCTCGATT CTACGTAGTA ATGAACTAGT CCGTAACTTG 1141 AAAGTATTTC GATTTCTTGG CTTTATATAT CTTGTGGAAA GGACGACTAC TGGGGTTACG 1201 GTAGGATCGT ACGCGTTAAG TCgacaatca acctctggat tacaaaattt gtgaaagatt