Construct: ORF TRCN0000469944
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009561.1_s317c1
- Derived from:
- ccsbBroadEn_14409
- DNA Barcode:
- TAGAATGGACATGCAGTAGCCTAT
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- FMC1 (154791)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469944
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 154791 | FMC1 | formation of mitochondrial ... | NM_197964.5 | 99.4% | 8delC;22T>G | |
| 2 | human | 154791 | FMC1 | formation of mitochondrial ... | NR_073059.1 | 27.7% | (many diffs) | |
| 3 | human | 154791 | FMC1 | formation of mitochondrial ... | NR_073058.1 | 27.4% | (many diffs) | |
| 4 | human | 154791 | FMC1 | formation of mitochondrial ... | NR_073060.1 | 27.1% | (many diffs) | |
| 5 | human | 100996928 | FMC1-LUC7L2 | FMC1-LUC7L2 readthrough | NM_001244584.2 | 22.4% | (many diffs) | |
| 6 | mouse | 66117 | Fmc1 | formation of mitochondrial ... | NM_025363.3 | 88.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 75
- ORF length:
- 9
- Sequence:
-
1 TCTTCCATTT CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGGC GGCTTAGGGT CCCCGGCGCA CACTTTTCGA GGACTTCTGC GGGAGTTGCG 121 CTACCTGAGC GCGGCCACCG GCCGACCCTA TCGCGACACC GCGGCCTATC GGTACCTTGT 181 GAAGGCTTTC CGTGCACATC GGGTCACCAG TGAAAAGTTG TGCAGAGCCC AACATGAGCT 241 TCATTTCCAA GCTGCCACCT ATCTCTGCCT CCTGCGTAGC ATCCGGAAAC ATGTGGCCCT 301 ACATCAGGAA TTTCATGGCA AGGGTGAGCG CTCGGTGGAG GAGTCTGCTG GCTTGGTGGG 361 TCTCAAGTTG CCCCATCAGC CTGGAGGGAA GGGCTGGGAG CCATGCCCAA CTTTCTTGTA 421 CAAAGTGGTT GATATCGGTA AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA 481 GTAATGAACT AGTCCGTAAC TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG 541 AAAGGACGAT AGAATGGACA TGCAGTAGCC TATACGCGTT AAGTCgacaa tcaacctctg 601 gattacaaaa tttgtgaaag att