Transcript: Human NM_001244584.2

Homo sapiens FMC1-LUC7L2 readthrough (FMC1-LUC7L2), mRNA.

Source:
NCBI, updated 2018-11-28
Taxon:
Homo sapiens (human)
Gene:
FMC1-LUC7L2 (100996928)
Length:
2747
CDS:
254..1630

Additional Resources:

NCBI RefSeq record:
NM_001244584.2
NBCI Gene record:
FMC1-LUC7L2 (100996928)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001244584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000314557 CCTACGGAGTTACGTACTATT pLKO_005 1664 3UTR 100% 13.200 6.600 Y LUC7L2 n/a
2 TRCN0000195117 CTCTATTCTGTTACGTCATTA pLKO.1 2510 3UTR 100% 13.200 6.600 Y LUC7L2 n/a
3 TRCN0000099566 GCACCTGGGATTTATTGAAAT pLKO.1 1093 CDS 100% 13.200 6.600 Y Luc7l2 n/a
4 TRCN0000309132 GCACCTGGGATTTATTGAAAT pLKO_005 1093 CDS 100% 13.200 6.600 Y Luc7l2 n/a
5 TRCN0000001190 GCTGTGGGCATTTCCTCTATT pLKO.1 2496 3UTR 100% 13.200 6.600 Y LUC7L2 n/a
6 TRCN0000165861 GTGGCCCTACATCAGGAATTT pLKO.1 482 CDS 100% 13.200 6.600 Y FMC1 n/a
7 TRCN0000001191 CGGTCCTATGAGAGTGCTAAT pLKO.1 1556 CDS 100% 10.800 5.400 Y LUC7L2 n/a
8 TRCN0000314556 CGGTCCTATGAGAGTGCTAAT pLKO_005 1556 CDS 100% 10.800 5.400 Y LUC7L2 n/a
9 TRCN0000163507 GCCCTACATCAGGAATTTCAT pLKO.1 485 CDS 100% 5.625 2.813 Y FMC1 n/a
10 TRCN0000330662 GCCCTACATCAGGAATTTCAT pLKO_005 485 CDS 100% 5.625 2.813 Y FMC1 n/a
11 TRCN0000165902 GAAACATGTGGCCCTACATCA pLKO.1 475 CDS 100% 4.950 2.475 Y FMC1 n/a
12 TRCN0000330661 GAAACATGTGGCCCTACATCA pLKO_005 475 CDS 100% 4.950 2.475 Y FMC1 n/a
13 TRCN0000001194 GAACGGCTGAAACGAAGAGAA pLKO.1 1175 CDS 100% 4.950 2.475 Y LUC7L2 n/a
14 TRCN0000320723 GAACGGCTGAAACGAAGAGAA pLKO_005 1175 CDS 100% 4.950 2.475 Y LUC7L2 n/a
15 TRCN0000001193 GTAATGGATGAAGTAGAGAAA pLKO.1 914 CDS 100% 4.950 2.475 Y LUC7L2 n/a
16 TRCN0000320721 GTAATGGATGAAGTAGAGAAA pLKO_005 914 CDS 100% 4.950 2.475 Y LUC7L2 n/a
17 TRCN0000250875 TTCCAAGCTGCCACCTATCTC pLKO_005 434 CDS 100% 4.950 2.475 Y Fmc1 n/a
18 TRCN0000001192 GCAGAGGAAGTTTATCGGAAT pLKO.1 956 CDS 100% 4.050 2.025 Y LUC7L2 n/a
19 TRCN0000320722 GCAGAGGAAGTTTATCGGAAT pLKO_005 956 CDS 100% 4.050 2.025 Y LUC7L2 n/a
20 TRCN0000162525 CAACATGAGCTTCATTTCCAA pLKO.1 419 CDS 100% 3.000 1.500 Y FMC1 n/a
21 TRCN0000164562 CCAACATGAGCTTCATTTCCA pLKO.1 418 CDS 100% 3.000 1.500 Y FMC1 n/a
22 TRCN0000196311 GAAGAATTAAAGAGAGTCGTA pLKO.1 1127 CDS 100% 2.640 1.320 Y LUC7L2 n/a
23 TRCN0000164105 CATCAGGAATTTCATGGCAAG pLKO.1 491 CDS 100% 2.250 1.125 Y FMC1 n/a
24 TRCN0000163964 CATGAGCTTCATTTCCAAGCT pLKO.1 422 CDS 100% 0.264 0.132 Y FMC1 n/a
25 TRCN0000330660 CATGAGCTTCATTTCCAAGCT pLKO_005 422 CDS 100% 0.264 0.132 Y FMC1 n/a
26 TRCN0000166479 CAGGAATTTCATGGCAAGGGT pLKO.1 494 CDS 100% 0.750 0.375 Y FMC1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001244584.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03349 pDONR223 100% 83.9% 81.1% None (many diffs) n/a
2 ccsbBroad304_03349 pLX_304 0% 83.9% 81.1% V5 (many diffs) n/a
3 TRCN0000472615 TCCGGGGCCGATTCGTTAGCTGAC pLX_317 45.2% 83.9% 81.1% V5 (many diffs) n/a
4 ccsbBroadEn_14409 pDONR223 100% 22.4% None (many diffs) n/a
5 ccsbBroad304_14409 pLX_304 0% 22.4% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000469944 TAGAATGGACATGCAGTAGCCTAT pLX_317 100% 22.4% V5 (not translated due to prior stop codon) (many diffs) n/a
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