Construct: ORF TRCN0000469952
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004931.1_s317c1
- Derived from:
- ccsbBroadEn_12568
- DNA Barcode:
- ACTACGCCCGTAATATCTTGTCGC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NKAIN1 (79570)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469952
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79570 | NKAIN1 | sodium/potassium transporti... | NM_024522.3 | 78.7% | 78.7% | 1_132del |
| 2 | human | 79570 | NKAIN1 | sodium/potassium transporti... | XM_017002320.1 | 65.7% | 65.7% | 1_132del;191_192ins81 |
| 3 | mouse | 67149 | Nkain1 | Na+/K+ transporting ATPase ... | NM_025998.3 | 72.7% | 78.7% | (many diffs) |
| 4 | mouse | 67149 | Nkain1 | Na+/K+ transporting ATPase ... | XM_006539116.3 | 70.4% | 76.1% | (many diffs) |
| 5 | mouse | 67149 | Nkain1 | Na+/K+ transporting ATPase ... | XM_006539117.3 | 69.3% | 75.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 558
- ORF length:
- 489
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtcatc ctgggcatct ttggcaccgt gcagtaccgc tcccggtacc 121 TCATCCTGTA TGCAGCCTGG CTGGTGCTCT GGGTTGGCTG GAATGCATTT ATCATCTGCT 181 TCTACTTGGA GGTTGGACAG CTGTCCCAGG ACCGGGACTT CATCATGACC TTCAACACAT 241 CCCTGCACCG CTCCTGGTGG ATGGAGAATG GGCCAGGCTG CCTGGTGACA CCTGTTCTGA 301 ACTCCCGCCT GGCTCTGGAG GACCACCATG TCATCTCTGT CACTGGCTGC CTGCTTGACT 361 ACCCCTACAT TGAAGCCCTC AGCAGCGCCC TGCAGATCTT CCTGGCACTG TTCGGCTTCG 421 TGTTCGCCTG CTACGTGAGC AAAGTGTTCC TGGAGGAGGA GGACAGCTTT GACTTCATCG 481 GCGGCTTTGA CTCCTACGGA TACCAGGCGC CCCAGAAGAC GTCGCATTTA CAGCTGCAGC 541 CTCTGTACAC GTCGGGGTTG CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 601 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 661 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGAACTACGC CCGTAATATC 721 TTGTCGCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt