Construct: ORF TRCN0000469958
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017673.1_s317c1
- Derived from:
- ccsbBroadEn_09040
- DNA Barcode:
- TCGGTTATCAAGTTAACCCTCTGT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MPIG6B (80739)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469958
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_138277.2 | 100% | 100% | |
| 2 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_025260.3 | 90.9% | 90.3% | (many diffs) |
| 3 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_138272.2 | 84.6% | 72.3% | (many diffs) |
| 4 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_011514921.2 | 81.8% | 78.3% | (many diffs) |
| 5 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_011514920.2 | 76.3% | 62.2% | (many diffs) |
| 6 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_138273.2 | 75% | 56.1% | (many diffs) |
| 7 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_138274.2 | 72.6% | 71.8% | (many diffs) |
| 8 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_017011332.1 | 67.3% | 47.1% | (many diffs) |
| 9 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_017011331.1 | 67.2% | 71.3% | (many diffs) |
| 10 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | NM_138275.2 | 67% | 60.5% | (many diffs) |
| 11 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_017011330.1 | 60.8% | 61.2% | (many diffs) |
| 12 | human | 80739 | MPIG6B | megakaryocyte and platelet ... | XM_017011333.1 | 60.8% | 50.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 735
- ORF length:
- 666
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggctgtgttt ctgcagctgc taccgctgct gctctcgagg gcccaaggga 121 accctggggc ttctctggac ggccgccctg gggaccgggt gaatctctcc tgcggaggag 181 tctctcatcc catccgctgg gtctgggcac ccagcttccc ggcctgcaag ggcctgtcca 241 aaggacgccg accgatcctg tgggcctctt cgagcgggac ccccaccgtg cctcccctcc 301 agcctttcgt cggccgccta cgctccctgg actcTGGTAT CCGGCGGCTG GAGCTCCTCT 361 TGAGCGCGGG GGACTCGGGC ACTTTTTTCT GCAAGGGCCG CCACGAGGAC GAGAGCCGTA 421 CAGTGCTTCA CGTGCTGGGG GACAGGACCT ATTGCAAGGC CCCCGGGCCT ACCCATGGGT 481 CCGTGTATCC CCAGCTCCTG ATCCCGCTGC TGGGCGCTGG GTTGGTGCTC GGACTGGGAG 541 CTTTGGGCCT GGTCTGGTGG CTGCACAGGC GCCTGCCCCC GCAACCGATT CGACCACTCC 601 CTAGATTTGC TCTGTCCCCC CCACATAGCT CCACTTGTGA AAACCGAGCC CCAGAGGCCA 661 GTAAAGGAGG AAGAGCCCAA GATTCCAGGG GACCTGGACC AGGAACCGGT AAGGGCATGG 721 GGATGGGAAG GGGATTGCCA ACTTTCTTGT ACAAAGTGGT TGATATCGGT AAGCCTATCC 781 CTAACCCTCT CCTCGGTCTC GATTCTACGT AGTAATGAAC TAGTCCGTAA CTTGGAAGTA 841 TTTCGATTTC TTGGCTTTAT ATATCTTGTG GAAAGGACGA TCGGTTATCA AGTTAACCCT 901 CTGTACGCGT TAAGTCgaca atcaacctct ggattacaaa atttgtgaaa gatt