Construct: ORF TRCN0000469975

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005046.1_s317c1
Derived from:
ccsbBroadEn_14367
DNA Barcode:
AAGGTTCTCCTTAATAGGATCCTG
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TTC8 (123016)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000469975
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 123016 TTC8 tetratricopeptide repeat do... NM_001288781.1 99.9% 99.2% 1581delT
2 human 123016 TTC8 tetratricopeptide repeat do... NM_198309.3 95% 94.3% 593_594ins78;1503delT
3 human 123016 TTC8 tetratricopeptide repeat do... XM_011536433.2 94.6% 93.9% 1393_1394ins84;1497delT
4 human 123016 TTC8 tetratricopeptide repeat do... XM_011536434.2 94.2% 93.5% 458_459ins90;1491delT
5 human 123016 TTC8 tetratricopeptide repeat do... NM_144596.3 93.2% 92.6% 112_141del;623_624ins78;1533delT
6 human 123016 TTC8 tetratricopeptide repeat do... NM_001366535.2 89.7% 89% 593_594ins78;1315_1316ins84;1419delT
7 human 123016 TTC8 tetratricopeptide repeat do... NM_198310.3 89.3% 88.7% 458_459ins90;503_504ins78;1413delT
8 human 123016 TTC8 tetratricopeptide repeat do... NM_001366536.2 84.1% 83.4% (many diffs)
9 human 123016 TTC8 tetratricopeptide repeat do... NM_001288782.1 58.9% 57.8% (many diffs)
10 human 123016 TTC8 tetratricopeptide repeat do... NM_001288783.1 51.9% 51.2% 0_1ins765;816delT
11 human 123016 TTC8 tetratricopeptide repeat do... XM_024449477.1 51.9% 51.2% 0_1ins765;816delT
12 human 123016 TTC8 tetratricopeptide repeat do... NR_159362.2 29% (many diffs)
13 mouse 76260 Ttc8 tetratricopeptide repeat do... NM_029553.3 84.3% 90.3% (many diffs)
14 mouse 76260 Ttc8 tetratricopeptide repeat do... NM_198311.1 82.8% 88.7% (many diffs)
15 mouse 76260 Ttc8 tetratricopeptide repeat do... XM_017315241.1 47% 49.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1659
ORF length:
1593
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatgag ctcggagatg gagccgctgc tcctggcctg gagctatttt aggcgcagga
121agttccagct ctgcgccgat ctatgcacgc agatgctgga gaagtcccct tatgaccagg
181cagcttggat cttaaaagca agagcgctaa cagaaatggt atacatagat gaaattgatg
241tagatcagga aggaattgca gaaatgatgc tggatgaaaa tgctatagct caagttccac
301gccctggaac gtctttgaaa ctccctggaa ctaatcagac aggagggcct agccaggccg
361ttaggccaat cacacaagct ggaagaccca ttacaggttt cctcaggccc agcacgcaga
421gtggaaggcc aggcactatg gaacaggcta tcagaacacc cagaaccgcc tacacagccc
481gccctatcac cagctcctcc ggaagatttg tcaggctggg aacggcttcc atgcttacaa
541gtcctgatgg accatttata aatttatcta ggctgaattt aacaaagtat tcccagaaac
601ctaagttggc aaaggctttg tttgagtata tctttcatca tgaaaatgat gttaagacta
661ttcatcttga agatgtagtt ctacatcttg gaatttaccc attcttattg aggaataaaa
721atcacattga aaaaaatgct ttggatctgg ctgccctctc cacagaacat tctcagtaca
781aggactggtg gtggaaagta cagattggaa aatgttacta caggttggga atgtatcgtg
841aagcagaaaa acagtttaaa tcagccctga agcagcagga aatggtagat acatttctgt
901acttggcaaa agtttatgtc tcattggatc aacctgtgac tgctttaaat cttttcaaac
961aaggcttaga taagtttcca ggagaagtaa ccctgctctg tggaattgca agaatctatg
1021aggaaatgaa caatatgtca tcagcagcag aatattacaa agaagttttg aaacaagaca
1081atactcatgt ggaagccatc gcatgcattg gaagcaacca cttctattct gatcagccag
1141aaatagctct ccggttttac aggcggctgc tgcagatggg catttataac ggccagcttt
1201ttaaCAATCT GGGGCTGTGT TGCTTCTATG CCCAGCAGTA TGATATGACT CTGACCTCAT
1261TTGAACGTGC CCTTTCTTTG GCTGAAAATG AAGAAGAGGC AGCTGATGTC TGGTACAACT
1321TGGGACATGT AGCTGTGGGA ATAGGAGATA CAAATTTGGC CCATCAGTGC TTCAGGCTGG
1381CTCTGGTCAA CAACAACAAC CACGCCGAGG CCTACAACAA CCTGGCTGTG CTGGAGATGC
1441GGAAGGGCCA CGTTGAACAG GCAAGGGCAC TATTACAAAC TGCATCATCA TTAGCACCCC
1501ATATGTATGA ACCGCATTTT AATTTTGCAA CAATCTCTGA TAAGATTGGA GATCTGCAGA
1561GAAGCTATGT TGCTGCGCAG AAGTCTGAAG CAGCATTTCC AGACCATGTG GACACACAAC
1621ATTTAATTAA ACAATTAAGG CAGCATTTGC TATGCTCTGC CCAACTTTCT TGTACAAAGT
1681GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG
1741AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA
1801CGAAAGGTTC TCCTTAATAG GATCCTGACG CGTTAAGTCg acaatcaacc tctggattac
1861aaaatttgtg aaagatt

Download FASTA (ORF) (Full)

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