Construct: ORF TRCN0000469976
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013176.1_s317c1
- Derived from:
- ccsbBroadEn_03617
- DNA Barcode:
- GGACTATCAACGTTTCATCCCGAA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FLVCR2 (55640)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469976
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 55640 | FLVCR2 | FLVCR heme transporter 2 | NM_017791.3 | 100% | 100% | |
| 2 | human | 55640 | FLVCR2 | FLVCR heme transporter 2 | NM_001195283.2 | 59% | 58.3% | (many diffs) |
| 3 | mouse | 217721 | Mfsd7c | major facilitator superfami... | NM_145447.2 | 82.7% | 82.8% | (many diffs) |
| 4 | mouse | 217721 | Mfsd7c | major facilitator superfami... | XM_006515709.2 | 45% | 45.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1644
- ORF length:
- 1578
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gaatgaaggt cccaaccagg aagagagcga tgacacccct gtgccggagt 121 ccgcactcca agcggacccc agcgtctcgg tccatcccag cgtctcggtc catcccagcg 181 tctccatcaa ccccagcgtc tctgtccacc ccagcagttc ggcccacccc agtgccttag 241 cccaacccag tggcttggct caccccagta gctcgggccc tgaggacctc agcgtgatca 301 aggtgagcag gcgccgttgg gccgtggtcc tggtgtttag ctgctactcc atgtgcaact 361 cctttcagtg gatccagtac ggctccatca ataacatctt catgcacttc tacggtgtca 421 gtgcctttgc cattgactgg ctgtccatgt gctacatgct gacttacatc cctctgctcc 481 tgccagtggc ttggctgctg gagaagttcg gcctgcgcac cattgctctc actggctcgg 541 ctctcaactg cctgggggcc tgggtgaagc tgggcagcct gaagccgcat ctctttccgg 601 tcaccgtggt gggccagctc atctgctctg tggcccaggt tttcatcctg ggcatgccct 661 cccgcatcgc ttccgtctgg ttcggggcta atgaggtttc aacagcctgc tccgtggctg 721 tctttggcaa tcagcttgga attgcgattg ggttcttggt ccctcctgtt ttggtaccca 781 acattgaaga ccgggacgag cttgcctacc acatcagcat catgttctat ataataggag 841 gtgtggccac tctcctcctc atccttgtca tcattgtgtt caaggagaaa cctaaatatc 901 cccccagcag ggcccaatcc ctgagctatg ccttgacctc tcctgatgcc tcatacttag 961 gttccatcgc ccggctcttc aaaaatctca actttgtgct gcttgtcatc acctatggtc 1021 tgaatgctgg tgctttttat gccttgtcca ctcttctgaa tcgcatggtg atctggcact 1081 acccggggga agaagtgaat gctggaagaa ttggcctgac gatcgtcatt gcaggaatgc 1141 ttggggctgt gatctcagga atctggctgg ataggtccaa aaccTACAAA GAGACAACCC 1201 TGGTAGTCTA TATCATGACA CTGGTGGGCA TGGTGGTGTA CACGTTTACC TTGAACCTGG 1261 GACACCTGTG GGTAGTGTTC ATCACTGCTG GCACAATGGG CTTCTTTATG ACTGGCTATC 1321 TCCCACTGGG ATTTGAGTTT GCTGTGGAGC TCACGTACCC AGAATCAGAA GGCATCTCCT 1381 CCGGCCTCCT CAACATATCT GCACAGGTAT TTGGGATCAT CTTTACCATC TCCCAGGGCC 1441 AGATTATTGA CAACTATGGA ACCAAGCCTG GGAACATCTT CCTGTGTGTG TTCCTTACTC 1501 TTGGAGCAGC CCTCACTGCA TTCATTAAGG CAGATCTCCG GAGACAGAAA GCAAACAAAG 1561 AAACTCTTGA GAACAAACTC CAAGAGGAGG AGGAGGAGAG CAACACCAGC AAAGTGCCCA 1621 CTGCTGTGTC AGAGGATCAT CTCTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1681 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1741 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAG GACTATCAAC 1801 GTTTCATCCC GAAACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1861 att