Transcript: Mouse NM_145447.2

Mus musculus major facilitator superfamily domain containing 7C (Mfsd7c), mRNA.

Source:
NCBI, updated 2017-05-28
Taxon:
Mus musculus (mouse)
Gene:
Mfsd7c (217721)
Length:
3435
CDS:
314..1969

Additional Resources:

NCBI RefSeq record:
NM_145447.2
NBCI Gene record:
Mfsd7c (217721)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_145447.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000098135 GCGAGAATCTTTGTCAGTTAT pLKO.1 2950 3UTR 100% 13.200 9.240 N Mfsd7c n/a
2 TRCN0000098139 CACAGGTATTTGGGATCGTTT pLKO.1 1719 CDS 100% 4.950 3.465 N Mfsd7c n/a
3 TRCN0000098137 CCCATCATAGTAGCTTGGTTA pLKO.1 579 CDS 100% 4.950 3.465 N Mfsd7c n/a
4 TRCN0000098136 CGGCTCCATCAATAACATCTT pLKO.1 697 CDS 100% 4.950 3.465 N Mfsd7c n/a
5 TRCN0000154023 CGGCTCCATCAATAACATCTT pLKO.1 697 CDS 100% 4.950 3.465 N FLVCR2 n/a
6 TRCN0000098138 GCTTTCATTAAGTCAGATCTT pLKO.1 1835 CDS 100% 4.950 3.465 N Mfsd7c n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_145447.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_03617 pDONR223 100% 82.7% 82.8% None (many diffs) n/a
2 ccsbBroad304_03617 pLX_304 0% 82.7% 82.8% V5 (many diffs) n/a
3 TRCN0000469976 GGACTATCAACGTTTCATCCCGAA pLX_317 29.4% 82.7% 82.8% V5 (many diffs) n/a
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