Construct: ORF TRCN0000469980
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005189.1_s317c1
- Derived from:
- ccsbBroadEn_07869
- DNA Barcode:
- ACCATAGATGCTGGCCTTCCTACA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SUN1 (23353)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469980
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001171946.2 | 99.8% | 99.6% | 610G>C |
| 2 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001171945.2 | 92.3% | 92% | 1_63del;673G>C |
| 3 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367637.1 | 44.2% | 39.5% | (many diffs) |
| 4 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367687.1 | 32.4% | 29.3% | (many diffs) |
| 5 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367665.1 | 32.4% | 28.3% | (many diffs) |
| 6 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367653.1 | 32.3% | 28.3% | (many diffs) |
| 7 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001171944.2 | 32.2% | 22.2% | (many diffs) |
| 8 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367702.1 | 32% | 28.7% | (many diffs) |
| 9 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367672.1 | 31.5% | 27.6% | (many diffs) |
| 10 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367642.1 | 31.3% | 29.2% | (many diffs) |
| 11 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367694.1 | 31.1% | 27.3% | (many diffs) |
| 12 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001130965.3 | 31% | 27.2% | (many diffs) |
| 13 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367633.1 | 31% | 27.2% | (many diffs) |
| 14 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367634.1 | 31% | 27.2% | (many diffs) |
| 15 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367704.1 | 31% | 27.9% | (many diffs) |
| 16 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367669.1 | 30.7% | 26.9% | (many diffs) |
| 17 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367685.1 | 29.9% | 27.8% | (many diffs) |
| 18 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367693.1 | 29.6% | 26.1% | (many diffs) |
| 19 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367675.1 | 29.3% | 26.5% | (many diffs) |
| 20 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367696.1 | 29.2% | 27.1% | (many diffs) |
| 21 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367688.1 | 29% | 26.3% | (many diffs) |
| 22 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367683.1 | 29% | 26.3% | (many diffs) |
| 23 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367640.1 | 28.9% | 26.2% | (many diffs) |
| 24 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367676.1 | 28.9% | 26.2% | (many diffs) |
| 25 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367682.1 | 28.8% | 26.1% | (many diffs) |
| 26 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367638.1 | 28.8% | 26.8% | (many diffs) |
| 27 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367664.1 | 28.8% | 26.8% | (many diffs) |
| 28 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367674.1 | 28.7% | 26% | (many diffs) |
| 29 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367643.1 | 28.6% | 25.2% | (many diffs) |
| 30 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367700.1 | 28.6% | 25.9% | (many diffs) |
| 31 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367697.1 | 28.6% | 25.2% | (many diffs) |
| 32 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367698.1 | 27.8% | 25.2% | (many diffs) |
| 33 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367641.1 | 27.8% | 25.2% | (many diffs) |
| 34 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367690.1 | 27.7% | 25.1% | (many diffs) |
| 35 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367699.1 | 27.7% | 25.1% | (many diffs) |
| 36 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367703.1 | 27.6% | 25% | (many diffs) |
| 37 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367677.1 | 27.6% | 25% | (many diffs) |
| 38 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367692.1 | 27.5% | 25% | (many diffs) |
| 39 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367678.1 | 26.7% | 24.2% | (many diffs) |
| 40 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367705.1 | 26.7% | 24.2% | (many diffs) |
| 41 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367662.1 | 25.7% | 21.9% | (many diffs) |
| 42 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_025154.6 | 25.2% | 23.3% | (many diffs) |
| 43 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367660.1 | 25% | 21.4% | (many diffs) |
| 44 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367686.1 | 24.9% | 22.8% | (many diffs) |
| 45 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367671.1 | 24.7% | 21.1% | (many diffs) |
| 46 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367648.1 | 24.7% | 21.7% | (many diffs) |
| 47 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367670.1 | 24.7% | 21.1% | (many diffs) |
| 48 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367651.1 | 24.3% | 21.2% | (many diffs) |
| 49 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367667.1 | 23.7% | 21.6% | (many diffs) |
| 50 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367647.1 | 23.6% | 20.2% | (many diffs) |
| 51 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367701.1 | 23.2% | 20.6% | (many diffs) |
| 52 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367644.1 | 23% | 20.3% | (many diffs) |
| 53 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367706.1 | 23% | 20.3% | (many diffs) |
| 54 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367680.1 | 22.9% | 20.3% | (many diffs) |
| 55 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367681.1 | 22.9% | 20.2% | (many diffs) |
| 56 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367679.1 | 22.8% | 20.9% | (many diffs) |
| 57 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367689.1 | 22.8% | 20.8% | (many diffs) |
| 58 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367668.1 | 22.7% | 19.4% | (many diffs) |
| 59 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367649.1 | 22.7% | 19.4% | (many diffs) |
| 60 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367646.1 | 22.2% | 19.6% | (many diffs) |
| 61 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367673.1 | 22.1% | 19.6% | (many diffs) |
| 62 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367645.1 | 22% | 20.1% | (many diffs) |
| 63 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367636.1 | 22% | 19.5% | (many diffs) |
| 64 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367684.1 | 21.9% | 19.4% | (many diffs) |
| 65 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367655.1 | 21.5% | 19% | (many diffs) |
| 66 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367691.1 | 21.3% | 18.8% | (many diffs) |
| 67 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367666.1 | 21.2% | 18.8% | (many diffs) |
| 68 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NM_001367695.1 | 21.2% | 19.3% | (many diffs) |
| 69 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NR_160281.1 | 18.2% | (many diffs) | |
| 70 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NR_160283.1 | 17.9% | (many diffs) | |
| 71 | human | 23353 | SUN1 | Sad1 and UNC84 domain conta... | NR_160282.1 | 16.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 837
- ORF length:
- 771
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgga tttttctcgg cttcacatgt acagtcctcc ccagtgtgtg ccggagaaca 121 cgggctacac gtatgcgctc agttccagct attcttcaga tgctctggat tttgagacgg 181 agcacaaatt ggaccctgta tttgattctc cacggatgtc ccgccgtagt ttgcgcctgg 241 ccacgacagc atgcaccctg ggggatggtg aggctgtggg tgccgacagc ggcaccagca 301 gcgctgtctc cctgaagaac cgagcggcca gaacaaCAAA ACAGCGCAGA AGCACAAACA 361 AATCAGCTTT TAGTATCAAC CACGTGTCAA GGCAGGTCAC GTCCTCTGGC GTCAGCCACG 421 GCGGCACTGT CAGCCTGCAG GATGCTGTGA CTCGACGGCC TCCTGTATTG GACGAGTCTT 481 GGATTCGTGA ACAGACCACA GTGGACCACT TCTGGGGTCT TGATGATGAT GGTGATCTTA 541 AAGGTGGAAA TAAAGCTGCC ATTCAGGGAA ACGGGGATGT GGGAGCCGCC GCCGCCACCG 601 CGCACAACGG CTTCTCCTGC AGCAACTGCA GCATGCTGTC CGAGCGCAAG GACGTGCTCA 661 CGGCGCACCC CGCGCCCCCC GGGCCCGTGT CGAGAGTTTA TTCTAGGGAC AGGAATCAAA 721 AATGTAAGTC TCAGTCCTTT AAAACTCAGA AAAAGGTGTG TTTTCCAAAT TTAATATTTC 781 CTTTCTGTAA GTCTCAGTGT CTGCACTATT TGTCTTGGAG ACTTAAAATT ATCCCTTGCC 841 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 901 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 961 ATATATCTTG TGGAAAGGAC GAACCATAGA TGCTGGCCTT CCTACAACGC GTTAAGTCga 1021 caatcaacct ctggattaca aaatttgtga aagatt