Construct: ORF TRCN0000469985
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002161.2_s317c1
- Derived from:
- ccsbBroadEn_14009
- DNA Barcode:
- TACGTTGGCCACCGGCCATATTTC
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MAP3K14 (9020)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469985
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 9020 | MAP3K14 | mitogen-activated protein k... | NM_003954.5 | 99.9% | 1.5% | 10delA;2706C>G |
| 2 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XM_011525441.2 | 99.9% | 1.5% | 10delA;2706C>G |
| 3 | human | 9020 | MAP3K14 | mitogen-activated protein k... | XR_934591.1 | 60.8% | (many diffs) | |
| 4 | mouse | 53859 | Map3k14 | mitogen-activated protein k... | NM_016896.3 | 83.6% | .5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 237
- ORF length:
- 171
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggc agtgtggaaa tggcctgccc aggtgcccct ggctcagcag tggggcagca 121 gaaggaactc cccaaagcca aggagaagac gccgccactg gggaagaaac agagctccgt 181 ctacaagctt gaggccgtgg agaagagccc tgtgttctgc ggaaagtggg agatcctgaa 241 tgacgtgatt accaagggca cagccaagga aggctccgag gcagggccag ctgccatctc 301 tatcatcgcc caggctgagt gtgagaatag ccaagagttc agccccacct tttcagaacg 361 cattttcatc gctgggtcca aacagtacag ccagtccgag agtcttgatc agatccccaa 421 caatgtggcc catgctacag agggcaaaat ggcccgtgtg tgttggaagg gaaagcgtcg 481 cagcaaagcc cggaagaaac ggaagaagaa gagctcaaag tccctggctc atgcaggagt 541 ggccttggcc aaacccctcc ccaggacccc tgagcaggag agctgcacca tcccagtgca 601 ggaggatgag tctccactcg gcgccccata tgttagaaac accccgcagt tcaccaagcc 661 tctgaaggaa ccaggccttg ggcaactctg ttttaagcag cttggcgagg gcctacggcc 721 ggctctgcct cgatcagaac tccacaaact gatcagcccc ttgcaatgtc tgaaccacgt 781 gtggaaactg caccaccccc aggacggagg ccccctgccc ctgcccacgc accccttccc 841 ctatagcaga ctgcctcatc ccttcccatt ccaccctctc cagccctgga aacctcaccc 901 tctggagtcc ttcctgggca aactggcctg tgtagacagc cagaaaccct tgcctgaccc 961 acacctgagc aaactggcct gtgtagacag tccaaagccc ctgcctggcc cacacctgga 1021 gcccagctgc ctgtctcgtg gtgcccatga gaagttttct gtggaggaat acctagtgca 1081 tgctctgcaa ggcagcgtga gctcaggcca ggcccacagc ctgaccagcc tggccaagac 1141 ctgggcagca aggggctcca gatcccggga gcccagcccc aaaactgagg acaacgaggg 1201 tgtcctgctc actgagaaac tcaagccagt ggattatgag taccgagaag aagtccactg 1261 ggccacgcac cagctccgcc tgggcagagg ctccttcgga gaggtgcaca ggatggagga 1321 caagcagact ggcttccagt gcgctgtcaa aaaggtgcgg ctggaagtat ttcgggcaga 1381 ggagctgatg gcatgtgcag gattgacctc acccagaatt gtccctttgt atggagctgt 1441 gagagaaggg ccttgggtca acatcttcat ggagctgctg gaaggtggct ccctgggcca 1501 gctggtcaag gagcagggct gtctcccaga ggaccgggcc ctgtactacc tgggccaggc 1561 cctggagggt ctggaatacc tccactcacg aaggattctg catggggacg tcaaagctga 1621 caacgtgctc ctgtccagcg atgggagcca cgcagccctc tgtgactttg gccatgctgt 1681 gtgtcttcaa cctgatggcc tgggaaagtc cttgctcaca ggggactaca tccctggcac 1741 agagacccac atggctccgg aggtggtgct gggcaggagc tgcgacgcca aggtggatgt 1801 ctggagcagc tgctgtatga tgctgcacat gctcaacggc tgccacccct ggactcagtt 1861 cttccgaggg ccgctctgcc tcaagattgc cagcgagcct ccgcctgtga gggagatccc 1921 accctcctgc gcccctctca cagcccaggc catccaagag gggctgagga aagagcccat 1981 ccaccgcgtg tctgcagcgg agctgggagg gaaggtgaac cgggcactac agcaagtggg 2041 aggtctgaag agcccttgga ggggagaata taaagaacca agacatccac cgccaaatca 2101 agccaattac caccagaccc tccatgccca gccgagagag ctttcgccaa gggccccagg 2161 gccccggcca gctgaggaga caacaggcag agcccctaag ctccagcctc ctctcccacc 2221 agagccccca gagccaaaca agtctcctcc cttgactttg agcaaggagg agtctgggat 2281 gtgggaaccc ttacctctgt cctccctgga gccagcccct gccagaaacc ccagctcacc 2341 agagcggaaa gcaaccgtcc cggagcagga actgcagcag ctggaaatag aattattcct 2401 caacagcctg tcccagccat tttctctgga ggagcaggag caaattctct cgtgcctcag 2461 catcgacagc ctctccctgt cggatgacag tgagaagaac ccatcaaagg cctctcaaag 2521 ctcgcgggac accctgagct caggcgtaca ctcctggagc agccaggccg aggctcgaag 2581 ctccagctgg aacatggtgc tggcccgggg gcggcccacc gacaccccaa gctatttcaa 2641 tggtgtgaaa gtccaaatac agtctcttaa tggtgaacac ctgcacatcc gggagttcca 2701 ccgggtcaaa gtgggagaca tcgccactgg catcagcagc cagatcccag ctgcagcctt 2761 cagcttggtg accaaagacg ggcagcctgt tcgctacgac atggaggtgc cagactcggg 2821 catcgacctg cagtgcacac tggcccctga tggcagcttc gcctggagct ggagggtcaa 2881 gcatggccag ctggagaaca ggCCCTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG 2941 TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA 3001 ACTTGAAAGT ATTTCGATTT CTTGGCTTTA TATATCTTGT GGAAAGGACG ATACGTTGGC 3061 CACCGGCCAT ATTTCACGCG TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa 3121 agatt