Construct: ORF TRCN0000469986
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012789.1_s317c1
- Derived from:
- ccsbBroadEn_11000
- DNA Barcode:
- CAGCTCGTTGATTAAAGCAGTTTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NONO (4841)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469986
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4841 | NONO | non-POU domain containing o... | NM_001145410.1 | 89% | 89% | 1_126del |
| 2 | human | 4841 | NONO | non-POU domain containing o... | NM_001145408.2 | 72.1% | 72.1% | 1_393del |
| 3 | human | 4841 | NONO | non-POU domain containing o... | NM_001145409.2 | 72.1% | 72.1% | 1_393del |
| 4 | human | 4841 | NONO | non-POU domain containing o... | NM_007363.5 | 72.1% | 72.1% | 1_393del |
| 5 | mouse | 53610 | Nono | non-POU-domain-containing, ... | NM_001252518.1 | 66.3% | 71.4% | (many diffs) |
| 6 | mouse | 53610 | Nono | non-POU-domain-containing, ... | NM_023144.2 | 66.3% | 71.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1086
- ORF length:
- 1020
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcc actccgtgga aagcagctgc gtgtgcgctt tgcctgccat agtgcatccc 121 ttacagttcg aaaccttcct cagtatgtgt ccaacgaact gctggaagaa gccttttctg 181 tgtttggcca ggtagagagg gctgtagtca ttgtggatga tcgaggaagg ccctcaggaa 241 aaggcattgt tgagttctca gggaagccag ctgctcggaa agctctggac agatgcagtg 301 aaggctcctt cctgctaacc acatttcctc gtcctgtgac tgtggagccc atggaccagt 361 tagatgatga agagggactt ccagagaagc tggttataaa aaaccagcaa tttcacaagg 421 aacgagagca gccacccaga tttgcacagc ctggctcctt tgagtatgaa tatgccatgc 481 gctggaaggc actcattgag atggagaagc agcagcagga ccaagtggac cgcaacatca 541 aggaggctcg tgagaagctg gagatggaga tggaagctgc acgccatgag caccaggtca 601 tgctaatgag acaggatttg atgaggcgcc aagaagaact tcggaggatg gaagagctgc 661 acaaccaaga ggtgcaaaaa cgaaagcaac tggagcTCAG GCAGGAGGAA GAGCGCAGGC 721 GCCGTGAAGA AGAGATGCGG CGGCAGCAAG AAGAAATGAT GCGGCGACAG CAGGAAGGAT 781 TCAAGGGAAC CTTCCCTGAT GCGAGAGAGC AGGAGATTCG GATGGGTCAG ATGGCTATGG 841 GAGGTGCTAT GGGCATAAAC AACAGAGGTG CCATGCCCCC TGCTCCTGTG CCAGCTGGTA 901 CCCCAGCTCC TCCAGGACCT GCCACTATGA TGCCGGATGG AACTTTGGGA TTGACCCCAC 961 CAACAACTGA ACGCTTTGGT CAGGCTGCTA CAATGGAAGG AATTGGGGCA ATTGGTGGAA 1021 CTCCTCCTGC ATTCAACCGT GCAGCTCCTG GAGCTGAATT TGCCCCAAAC AAACGTCGCC 1081 GATACTACCC AACTTTCTTG TACAAAGTGG TTGATATCGG TAAGCCTATC CCTAACCCTC 1141 TCCTCGGTCT CGATTCTACG TAGTAATGAA CTAGTCCGTA ACTTGAAAGT ATTTCGATTT 1201 CTTGGCTTTA TATATCTTGT GGAAAGGACG ACAGCTCGTT GATTAAAGCA GTTTTACGCG 1261 TTAAGTCgac aatcaacctc tggattacaa aatttgtgaa agatt