Construct: ORF TRCN0000469989
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF018942.3_s317c1
- Derived from:
- ccsbBroadEn_14814
- DNA Barcode:
- AGTGTTCCTGGCGGATCAAGGCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PRKY (5616)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000469989
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 5613 | PRKX | protein kinase X-linked | NM_005044.5 | 73.7% | 70.1% | (many diffs) |
| 2 | human | 5616 | PRKY | protein kinase Y-linked (ps... | NR_028062.1 | 11.4% | 1_341del;451G>N;1173_7240del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 900
- ORF length:
- 831
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaggcgccc gggccggccc aggcggccgc ggcggagagc aactcccgag 121 aggtgacgga ggatgccgcc gactgggcgc ccgcgctctg ccccagcccc gaggcgcngt 181 cgccggaggc gcctgcctac cgcctgcagg actgcgacgc gctggtcacc atgggcactg 241 ggacgttcgg gcgggtgcac ctggtgaagg agaagacagc caagcatttc ttcgccctca 301 aggtgatgag cattcccgac gtcatccgcc ggaagcagga gcagcacgtg cacaatgaga 361 agtctgtcct gaaggaagtc agccacccgt tcctcatcag gctgttctgg acgtggcatg 421 aggagcgctt cctctacatg ctcatggagt atgtgccggg tggcgagctc ttcagctacc 481 tgcgcaaccg ggggcacttc tccagcacca cggggctctt ctactctgcg gagatcatct 541 gtgccattga gtacctgcac tccaaggaga tcgtctacag ggatttGAAG CCGGAGAACA 601 TCCTGCTGGA TAGGGATGGT CACATCAAGC TCACGGACTT TGGGTTTGCC AAGAAGCTGG 661 TAGACAGGAC TTGGACCCTC TGTGGAACAC CCGAGTACCT AGCCCCCGAA GTCATTCAGA 721 GCAAGGGCCA CGGAAGGGCC GTGGACTGGT GGGCCCTCGG CATCCTGATA TTCGAGATGC 781 TTTCGGGGTT TCCTCCATTT TTTGATGACA ACCCGTTTGG CATTTATCAG AAAATTCTTG 841 CAGGCAAACT ATATTTCCCC AGACATTTGG ATTTCCATGT AAAAACGGGG CGAATGATGT 901 TGCCAACTTT CTTGTACAAA GTGGTTGATA TCGGTAAGCC TATCCCTAAC CCTCTCCTCG 961 GTCTCGATTC TACGTAGTAA TGAACTAGTC CGTAACTTGA AAGTATTTCG ATTTCTTGGC 1021 TTTATATATC TTGTGGAAAG GACGAAGTGT TCCTGGCGGA TCAAGGCCAA CGCGTTAAGT 1081 Cgacaatcaa cctctggatt acaaaatttg tgaaagatt