Construct: ORF TRCN0000470005
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017570.1_s317c1
- Derived from:
- ccsbBroadEn_03221
- DNA Barcode:
- GATGCGGTGTGACGGATGTTCCCA
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GOLGA7 (51125)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470005
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 51125 | GOLGA7 | golgin A7 | NM_001174124.1 | 100% | 100% | |
| 2 | human | 51125 | GOLGA7 | golgin A7 | NM_001002296.2 | 95.4% | 89% | 366_367insTTTAG;398_411del |
| 3 | human | 51125 | GOLGA7 | golgin A7 | NM_001362979.1 | 95.4% | 89% | 366_367insTTTAG;398_411del |
| 4 | human | 51125 | GOLGA7 | golgin A7 | NM_001362980.1 | 95.4% | 89% | 366_367insTTTAG;398_411del |
| 5 | human | 51125 | GOLGA7 | golgin A7 | NM_016099.2 | 95.4% | 89% | 366_367insTTTAG;398_411del |
| 6 | human | 51125 | GOLGA7 | golgin A7 | NR_156426.1 | 20.7% | (many diffs) | |
| 7 | human | 51125 | GOLGA7 | golgin A7 | NR_156425.1 | 20.6% | (many diffs) | |
| 8 | human | 51125 | GOLGA7 | golgin A7 | NR_156427.1 | 17.7% | (many diffs) | |
| 9 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | XM_006509179.3 | 92.2% | 97.7% | (many diffs) |
| 10 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | NM_001042484.1 | 88.7% | 89% | (many diffs) |
| 11 | mouse | 57437 | Golga7 | golgi autoantigen, golgin s... | NM_020585.2 | 88.7% | 89% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 468
- ORF length:
- 402
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgag gccgcagcag gcgccggtgt ccggaaaggt gttcattcag cgagactaca 121 gcagtggcac acgctgccag ttccagacca agttccctgc ggagctggag aaccggattg 181 ataggcagca gtttGAAGAA ACAGTTCGAA CTCTAAATAA CCTTTATGCA GAAGCAGAGA 241 AGCTCGGCGG CCAGTCATAT CTCGAAGGTT GTTTGGCTTG TTTAACAGCA TATACCATCT 301 TCCTATGCAT GGAAACTCAT TATGAGAAGG TTCTGAAGAA AGTCTCCAAA TACATTCAAG 361 AGCAGAATGA GAAGATCTAT GCTCCACAAG GCCTCCTCCT GACAGACCCT ATTGAGCGAG 421 GACTGCGAGT TTTTAGATTG AAATTACCAT TTATGAAGAC AGAGGCATGC TCAACTTTCT 481 TGTACAAAGT GGTTGATATC GGTAAGCCTA TCCCTAACCC TCTCCTCGGT CTCGATTCTA 541 CGTAGTAATG AACTAGTCCG TAACTTGAAA GTATTTCGAT TTCTTGGCTT TATATATCTT 601 GTGGAAAGGA CGAGATGCGG TGTGACGGAT GTTCCCAACG CGTTAAGTCg acaatcaacc 661 tctggattac aaaatttgtg aaagatt