Construct: ORF TRCN0000470013
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007546.1_s317c1
- Derived from:
- ccsbBroadEn_09416
- DNA Barcode:
- AAACCACTCCTAACGATAACTAAC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCMTD1 (115294)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470013
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 115294 | PCMTD1 | protein-L-isoaspartate (D-a... | NM_052937.4 | 99.8% | 99.7% | 507G>A;935A>T |
| 2 | human | 115294 | PCMTD1 | protein-L-isoaspartate (D-a... | NM_001286782.1 | 67% | 59.5% | (many diffs) |
| 3 | human | 115294 | PCMTD1 | protein-L-isoaspartate (D-a... | NM_001363193.1 | 66.3% | 65.8% | (many diffs) |
| 4 | human | 115294 | PCMTD1 | protein-L-isoaspartate (D-a... | NM_001286783.2 | 50.6% | 50.4% | 0_1ins528;407A>T |
| 5 | human | 115294 | PCMTD1 | protein-L-isoaspartate (D-a... | XR_001745466.2 | 24.8% | (many diffs) | |
| 6 | mouse | 319263 | Pcmtd1 | protein-L-isoaspartate (D-a... | NM_183028.3 | 92.6% | 98.5% | (many diffs) |
| 7 | mouse | 319263 | Pcmtd1 | protein-L-isoaspartate (D-a... | XM_006495535.2 | 45.8% | 49.2% | (many diffs) |
| 8 | mouse | 319263 | Pcmtd1 | protein-L-isoaspartate (D-a... | XM_006495536.3 | 45.8% | 49.2% | (many diffs) |
| 9 | mouse | 319263 | Pcmtd1 | protein-L-isoaspartate (D-a... | XR_373188.2 | 18.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1137
- ORF length:
- 1071
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg aggagctgtg agtgctgggg aagataatga tgacttaatt gataatttaa 121 aagaagctca gtatattcgt actgaaagag tggagcaagc cttcagagcg attgatcgtg 181 gagattacta tttggaaggc tacagagaca atgcttacaa agacttagcc tggaagcatg 241 gaaacatcca cttgtcagca ccttgcattt attctgaagt tatggaagca ttgaaacttc 301 aaccaggatt gtcttttctt aacctgggaa gtggaaccgg atatttaagt acaatggtgg 361 gcttaatttt aggtcctttt ggaataaatc atgggattga gcttcattca gatgtggtgg 421 aatatgccaa ggaaaaactg gagagcttca tcaaaaatag tgatagcttt gataaatttg 481 agttctgtga acctgcattt gttgttggta attgcctcca gatagcttct gacagtcatc 541 agtatgatcg aatttattgt ggagctggag tacagaaaga ccatgaaaac tacatgaaaa 601 tattactaaa agttggaggc atattagtca tgcctataga ggatcagtta acacagatta 661 tgcgaactgg acagaacact tgggaaagta aaaatatcct tgctgtttca tttgctccac 721 ttgtgcaacc aagtaagaat gataatggca aaccagattc tgtgggactc cctccctgtg 781 ctgtcaggaa tctacaggac ttggctcgta tttacattcg acgcacactt agaaattTCA 841 TAAATGATGA GATGCAGGCC AAGGGGATTC CTCAAAGGGC TCCACCCAAA AGGAAAAGAA 901 AGAGAGTTAA ACAGAGAATT AACACTTACG TATTTGTGGG TAATCAGCTT ATTCCTCAGC 961 CTCTAGACAG TGAAGAGGAT GAAAAAATGG AAGAGGATAT CAAAGAAGAG GAGGAAAAAG 1021 ATCACAATGA AGCAATGAAG CCAGAGGAGC CACCTCAAAA TTTACTGAGA GAAAAAATCA 1081 TGAAGCTGCC CCTCCCTGAA TCTTTAAAAG CTTACTTGAC ATATTTTAGA GACAAATACC 1141 CAACTTTCTT GTACAAAGTG GTTGATATCG GTAAGCCTAT CCCTAACCCT CTCCTCGGTC 1201 TCGATTCTAC GTAGTAATGA ACTAGTCCGT AACTTGAAAG TATTTCGATT TCTTGGCTTT 1261 ATATATCTTG TGGAAAGGAC GAAAACCACT CCTAACGATA ACTAACACGC GTTAAGTCga 1321 caatcaacct ctggattaca aaatttgtga aagatt