Construct: ORF TRCN0000470018
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002991.1_s317c1
- Derived from:
- ccsbBroadEn_10206
- DNA Barcode:
- TATAGGTGATCGAGTCATGACCCC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCT3 (7203)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470018
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 7203 | CCT3 | chaperonin containing TCP1 ... | NM_005998.5 | 99.8% | 99.8% | 1_3delATG |
2 | human | 7203 | CCT3 | chaperonin containing TCP1 ... | NM_001008800.2 | 92.8% | 92.8% | 1_3delATG;91_92ins114 |
3 | human | 7203 | CCT3 | chaperonin containing TCP1 ... | NR_036565.1 | 73.5% | 1_234del;263_375del;1980_2220del | |
4 | human | 7203 | CCT3 | chaperonin containing TCP1 ... | NR_036564.1 | 71.9% | 1_234del;263_424del;2029_2269del | |
5 | mouse | 12462 | Cct3 | chaperonin containing Tcp1,... | NM_009836.1 | 89.6% | 97% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1698
- ORF length:
- 1632
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggg ccatcgtcca gtgctcgtgc tcagccagaa cacaaagcgt gaatccggaa 121 gaaaagttca atctggaaac atcaatgctg ccaagactat tgcagatatc atccgaacat 181 gtttgggacc caagtccatg atgaagatgc ttttggaccc aatgggaggc attgtgatga 241 ccaatgatgg caatgccatt cttcgagaga ttcaagtcca gcatccagcg gccaagtcca 301 tgatcgaaat tagccggacc caggatgaag aggttggaga tgggaccaca tcagtaatta 361 ttcttgcagg ggaaatgctg tctgtagctg agcacttcct ggagcagcag atgcacccaa 421 cagtggtgat cagtgcttac cgcaaggcat tggatgatat gatcagcacc ctaaagaaaa 481 taagtatccc agtcgacatc agtgacagtg atatgatgct gaacatcatc aacagctcta 541 ttactaccaa agccatcagt cggtggtcat ctttggcttg caacattgcc ctggatgctg 601 tcaagatggt acagtttgag gagaatggtc ggaaagagat tgacataaaa aaatatgcaa 661 gagtggaaaa gatacctgga ggcatcattg aagactcctg tgtcttgcgt ggagtcatga 721 ttaacaagga tgtgacccat ccacgtatgc ggcgctatat caagaaccct cgcattgtgc 781 tgctggattc ttctctggaa tacaagaaag gagaaagcca gactgacatt gagattacac 841 gagaggagga cttcacccga attctccaga tggaggaaga gtacatccag cagctctgtg 901 aggacattat ccaactgaag cccgatgtgg tcatcactga aaagggcatc tcagatttag 961 ctcagcacta ccttatgcgg gccaatatca cagccatccg cagagtccgg aagacagaca 1021 ataatcgcat tgctagagcc tgtggggccc ggatagtcag ccgaccagag gaactgagag 1081 aagatgatgt tggaacagga gcaggcctgt tggaaatcaa gaaaattgga gatgaatact 1141 ttactttcat cactgactgc aaagacccca aggcctgcac cattctcctc cggggggcta 1201 gcaaagagat tctctcggaa gtagaacgca acctccagga tgccatgcaa gtgtgtcgca 1261 atgttctcct ggaccctcag ctggtgccag ggggtggggc cTCCGAGATG GCTGTGGCCC 1321 ATGCCTTGAC AGAAAAATCC AAGGCCATGA CTGGTGTGGA ACAATGGCCA TACAGGGCTG 1381 TTGCCCAGGC CCTAGAGGTC ATTCCTCGTA CCCTGATCCA GAACTGTGGG GCCAGCACCA 1441 TCCGTCTACT TACCTCCCTT CGGGCCAAGC ACACCCAGGA GAACTGTGAG ACCTGGGGTG 1501 TAAATGGTGA GACGGGTACT TTGGTGGACA TGAAGGAACT GGGCATATGG GAGCCATTGG 1561 CTGTGAAGCT GCAGACTTAT AAGACAGCAG TGGAGACGGC AGTTCTGCTA CTGCGAATTG 1621 ATGACATCGT TTCAGGCCAC AAAAAGAAAG GCGATGACCA GAGCCGGCAA GGCGGGGCTC 1681 CTGATGCTGG CCAGGAGTGC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 1741 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 1801 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGATATAGGT GATCGAGTCA 1861 TGACCCCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt