Construct: ORF TRCN0000470059
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013434.1_s317c1
- Derived from:
- ccsbBroadEn_00515
- DNA Barcode:
- CAAATAGACCACTTGCACTGCATC
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ERH (2079)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470059
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2079 | ERH | ERH mRNA splicing and mitos... | NM_004450.3 | 100% | 100% | |
| 2 | mouse | 13877 | Erh | enhancer of rudimentary hom... | NM_007951.4 | 92.9% | 100% | (many diffs) |
| 3 | mouse | 13877 | Erh | enhancer of rudimentary hom... | NM_001347409.1 | 78.5% | 84.5% | (many diffs) |
| 4 | mouse | 13877 | Erh | enhancer of rudimentary hom... | XM_011243995.2 | 66.6% | 71.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 tcttccattt CAGGTGTCGT GAGGCTAGCA TCGATTGATC AACAAGTTTG TACAAAAAAG 61 TTGGCATGTC TCACACCATT TTGCTGGTAC AGCCTACCAA GAGGCCAGAA GGCAGAACTT 121 ATGCTGACTA CGAATCTGTG AATGAATGCA TGGAAGGTGT TTGTAAAATG TATGAAGAAC 181 ATCTGAAAAG AATGAATCCC AACAGTCCCT CTATCACATA TGACATCAGT CAGTTGTTTG 241 ATTTCATCGA TGATCTGGCA GACCTCAGCT GCCTGGTTTA CCGAGCTGAT ACCCAGACAT 301 ACCAGCCTTA TAACAAAGAC TGGATTAAAG AGAAGATCTA CGTGCTCCTT CGTCGGCAGG 361 CCCAACAGGC TGGGAAATAC CCAACTTTCT TGTACAAAGT GGTTGATATC GGTAAGCCTA 421 TCCCTAACCC TCTCCTCGGT CTCGATTCTA CGTAGTAATG AACTAGTCCG TAACTTGAAA 481 GTATTTCGAT TTCTTGGCTT TATATATCTT GTGGAAAGGA CGACAAATAG ACCACTTGCA 541 CTGCATCACG CGTTAAGTCg acaatcaacc tctggattac aaaatttgtg aaagatt