Construct: ORF TRCN0000470065
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006110.1_s317c1
- Derived from:
- ccsbBroadEn_04664
- DNA Barcode:
- ACGGAACTTAACTCCTAGGGTGTT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- OSBPL5 (114879)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470065
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 114879 | OSBPL5 | oxysterol binding protein l... | NM_020896.4 | 100% | 100% | |
| 2 | human | 114879 | OSBPL5 | oxysterol binding protein l... | XM_011519873.3 | 100% | 100% | |
| 3 | human | 114879 | OSBPL5 | oxysterol binding protein l... | XM_017017163.2 | 100% | 100% | |
| 4 | human | 114879 | OSBPL5 | oxysterol binding protein l... | NM_001144063.2 | 92.2% | 92.2% | 397_398ins204 |
| 5 | human | 114879 | OSBPL5 | oxysterol binding protein l... | NM_145638.3 | 92.2% | 92.2% | 397_398ins204 |
| 6 | human | 114879 | OSBPL5 | oxysterol binding protein l... | XM_017017164.1 | 57.9% | 57.9% | 0_1ins1110 |
| 7 | mouse | 79196 | Osbpl5 | oxysterol binding protein-l... | XM_006508659.3 | 78.3% | 80.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2703
- ORF length:
- 2637
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggaggaggcc ttcctccggc gccgcttctc cctgtgtcca ccttcctcca 121 cccctcagaa agtcgacccc cggaagctca cccggaactt gctcctcagc ggagacaatg 181 agctctaccc actcagccca gggaaggaca tggagcccaa cggcccgtcg ctgcccaggg 241 atgaagggcc cccgacccca agctctgcca cgaaggtgcc accggcagag tacaggctgt 301 gcaacgggtc agacaaggaa tgtgtgtccc ccaccgccag ggtcaccaag aaggagactc 361 tcaaggcgca gaaggagaac taccggcagg agaagaagcg cgccacacgg cagctgctca 421 gcgctctgac agaccccagc gtggtcatca tggctgacag cctgaagatc cgcggcaccc 481 tgaagagctg gaccaagctg tggtgcgtgc tgaagccggg ggtgctgctc atctacaaga 541 cgcccaaggt gggccagtgg gtgggcacgg tgctgctgca ctgctgcgag ctcatcgagc 601 ggccctccaa gaaggacggc ttctgcttca agctcttcca cccgctggat cagtccgtct 661 gggccgtgaa gggccccaaa ggtgagagcg tgggctccat cacacagccc ctgcccagca 721 gctacctgat cttcagggcc gcctccgagt cagatggtcg ctgctggctg gacgccctgg 781 agctggccct gcgctgctct agcctactga gactgggcac ctgcaagccg ggccgagacg 841 gggagccagg gacctcgcca gacgcatcac cctcatcgct ctgtgggctg ccagcctcag 901 ccaccgtcca cccagaccaa gacctgttcc cactgaacgg gtcttccctg gagaacgatg 961 cattctcaga caagtcggag agagagaacc ctgaggagtc agataccgag acccaggacc 1021 atagccggaa gacggagagt ggcagcgacc agtcagagac ccctggggcc ccggtgcgga 1081 gagggaccac ctatgtggag caggtccagg aggagctggg ggagctgggc gaggcgtccc 1141 aggtggagac agtgtcagag gagaacaaga gtctgatgtg gaccctgctg aagcagctac 1201 ggccaggcat ggacctgtcc cgcgtggtgc tacccacgtt cgtactggag ccgcgctcct 1261 tcctgaacaa gctctccgac tactactacc acgcagacct gctctccagg gctgcggtgg 1321 aggaggatgc ctacagccgc atgaagctgg tgctgcggtg gtacctgtct ggcttctaca 1381 agaagcccaa gggaatcaag aagccgtaca accccatcct gggggagacc ttccgctgct 1441 gctggttcca cccgcagact gacagccgca cattctacat agcagagcag gtgtcccacc 1501 acccgcccgt gtctgccttc cacgtcagca accggaagga cggcttctgc atcagtggca 1561 gcatcacagc caagtccagg ttttatggga actcgctgtc ggcgctgctg gacggcaaag 1621 ccacgctcac cttcctgaac cgagccgagg attacaccct taccatgccc tacgcccact 1681 gcaaaggaat cctgtatggc acgatgaccc tggagctggg tgggaaggtc accatcgagt 1741 gtgcgaagaa caacttccag gcccagctgg aattcaaact caagcccttc ttcgggggta 1801 gcaccagcat caaccagatc tcgggaaaga tcacgtcggg agaggaagtc ctggcgagcc 1861 tcagtggcca ctgggacagg gacgtgttta tcaaggagga agggagcgga agcagtgcgc 1921 ttttctggac cccgagcggg gaggtccgca gacagaggct gaggcagcac acggtgccgc 1981 tggaggagca gacggagctg gagtccgaga ggctctggca gcacgtcacc agggccatca 2041 gcaagggcga ccagcacagg gccacacagg agaagtttgc actggaggag gcacagcggc 2101 agcgggcccg tgagcggcag gagagcctca tgccctggaa gccgcagctg ttccacctgg 2161 accccatcac ccaggagtgg cactaccgat acgaggacca cagcccctgg gaccccctga 2221 aggacatcgc ccagtttgag caagacggga tcctgcggac cttgcagcag gaggccgtgg 2281 cccgccagac caccTTCCTG GGCAGCCCAG GGCCCAGGCA CGAGAGGTCT GGCCCAGACC 2341 AGCGGCTTCG CAAGGCCAGC GACCAGCCCT CCGGCCACAG CCAGGCCACG GAGAGCAGCG 2401 GATCCACGCC TGAGTCCTGC CCAGAGCTCT CAGACGAGGA GCAGGATGGT GACTTTGTCC 2461 CTGGCGGTGA GAGCCCATGC CCTCGGTGCA GGAAGGAGGC GCGGCGGCTG CAGGCCCTGC 2521 ACGAGGCCAT CCTCTCCATC CGAGAGGCCC AGCAGGAGCT GCACAGGCAC CTCTCGGCCA 2581 TGCTGAGCTC CACGGCACGG GCAGCACAGG CACCGACCCC AGGCCTCCTG CAGAGCCCCC 2641 GATCCTGGTT CCTGCTCTGC GTGTTCCTGG CGTGTCAGCT GTTCATTAAC CACATCCTCA 2701 AATACCCAAC TTTCTTGTAC AAAGTGGTTG ATATCGGTAA GCCTATCCCT AACCCTCTCC 2761 TCGGTCTCGA TTCTACGTAG TAATGAACTA GTCCGTAACT TGAAAGTATT TCGATTTCTT 2821 GGCTTTATAT ATCTTGTGGA AAGGACGAAC GGAACTTAAC TCCTAGGGTG TTACGCGTTA 2881 AGTCgacaat caacctctgg attacaaaat ttgtgaaaga tt