Construct: ORF TRCN0000470072

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF005591.1_s317c1
Derived from:
ccsbBroadEn_02330
DNA Barcode:
TAGACTACAACTTGTACGTGCAAT
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
G3BP1 (10146)

Vector Information:

Vector Backbone:
pLX_317
Pol II Cassette 1:
SV40-PuroR
Pol II Cassette 2:
EF1a-TRCN0000470072
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 10146 G3BP1 G3BP stress granule assembl... NM_005754.3 100% 100%
2 human 10146 G3BP1 G3BP stress granule assembl... NM_198395.2 100% 100%
3 mouse 27041 G3bp1 GTPase activating protein (... NM_013716.2 86% 93.3% (many diffs)
4 mouse 27041 G3bp1 GTPase activating protein (... XM_006533444.2 73.3% 79.6% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1464
ORF length:
1398
Sequence:
1tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag
61ttggcatggt gatggagaag cctagtcccc tgctggtcgg gcgggaattt gtgagacagt
121attacacact gctgaaccag gccccagaca tgctgcatag attttatgga aagaactctt
181cttatgtcca tgggggattg gattcaaatg gaaagccagc agatgcagtc tacggacaga
241aagaaatcca caggaaagtg atgtcacaaa acttcaccaa ctgccacacc aagattcgcc
301atgttgatgc tcatgccacg ctaaatgatg gtgtggtagt ccaggtgatg gggcttctct
361ctaacaacaa ccaggctttg aggagattca tgcaaacgtt tgtccttgct cctgaggggt
421ctgttgcaaa taaattctat gttcacaatg atatcttcag ataccaagat gaggtctttg
481gtgggtttgt cactgagcct caggaggagt ctgaagaaga agtagaggaa cctgaagaaa
541gacagcaaac acctgaggtg gtacctgatg attctggaac tttctatgat caggcagttg
601tcagtaatga catggaagaa catttagagg agcctgttgc tgaaccagag cctgatcctg
661aaccagaacc agaacaagaa cctgtatctg aaatccaaga ggaaaagcct gagccagtat
721tagaagaaac tgcccctgag gatgctcaga agagttcttc tccagcacct gcagacatag
781ctcagacagt acaggaagac ttgaggacat tttcttgggc atctgtgacc agtaagaatc
841ttccacccag tggagctgtt ccagttactg ggataccacc tcatgttgtt aaagtaccag
901cttcacagcc ccgtccagag tctaagcctg aatctcagat tccaccacaa agacctcagc
961gggatcaaag agtgcgagaa caacgaataa atattcctcc ccaaagggga cccagaccaa
1021tccgtgaggc tggtgagcaa ggtgacattg aaccccgaag aatggtgaga caccctgaca
1081gtcaccaact cttcattggc aacctgcctc atgaagtgga caaatcagag cttaaagatt
1141tctttcaaag ttatggaaac gtggtggagt tgcgcattaa cagtggtggg aaattacCCA
1201ATTTTGGTTT TGTTGTGTTT GATGATTCTG AGCCTGTTCA GAAAGTCCTT AGCAACAGGC
1261CCATCATGTT CAGAGGTGAG GTCCGTCTGA ATGTCGAAGA GAAGAAGACT CGAGCTGCCA
1321GGGAAGGCGA CCGACGAGAT AATCGCCTTC GGGGACCTGG AGGCCCTCGA GGTGGGCTGG
1381GTGGTGGAAT GAGAGGCCCT CCCCGTGGAG GCATGGTGCA GAAACCAGGA TTTGGAGTGG
1441GAAGGGGGCT TGCGCCACGG CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA
1501AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC
1561TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGACTACAAC
1621TTGTACGTGC AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag
1681att

Download FASTA (ORF) (Full)

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