Construct: ORF TRCN0000470072
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005591.1_s317c1
- Derived from:
- ccsbBroadEn_02330
- DNA Barcode:
- TAGACTACAACTTGTACGTGCAAT
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- G3BP1 (10146)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470072
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10146 | G3BP1 | G3BP stress granule assembl... | NM_005754.3 | 100% | 100% | |
| 2 | human | 10146 | G3BP1 | G3BP stress granule assembl... | NM_198395.2 | 100% | 100% | |
| 3 | mouse | 27041 | G3bp1 | GTPase activating protein (... | NM_013716.2 | 86% | 93.3% | (many diffs) |
| 4 | mouse | 27041 | G3bp1 | GTPase activating protein (... | XM_006533444.2 | 73.3% | 79.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1464
- ORF length:
- 1398
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatggt gatggagaag cctagtcccc tgctggtcgg gcgggaattt gtgagacagt 121 attacacact gctgaaccag gccccagaca tgctgcatag attttatgga aagaactctt 181 cttatgtcca tgggggattg gattcaaatg gaaagccagc agatgcagtc tacggacaga 241 aagaaatcca caggaaagtg atgtcacaaa acttcaccaa ctgccacacc aagattcgcc 301 atgttgatgc tcatgccacg ctaaatgatg gtgtggtagt ccaggtgatg gggcttctct 361 ctaacaacaa ccaggctttg aggagattca tgcaaacgtt tgtccttgct cctgaggggt 421 ctgttgcaaa taaattctat gttcacaatg atatcttcag ataccaagat gaggtctttg 481 gtgggtttgt cactgagcct caggaggagt ctgaagaaga agtagaggaa cctgaagaaa 541 gacagcaaac acctgaggtg gtacctgatg attctggaac tttctatgat caggcagttg 601 tcagtaatga catggaagaa catttagagg agcctgttgc tgaaccagag cctgatcctg 661 aaccagaacc agaacaagaa cctgtatctg aaatccaaga ggaaaagcct gagccagtat 721 tagaagaaac tgcccctgag gatgctcaga agagttcttc tccagcacct gcagacatag 781 ctcagacagt acaggaagac ttgaggacat tttcttgggc atctgtgacc agtaagaatc 841 ttccacccag tggagctgtt ccagttactg ggataccacc tcatgttgtt aaagtaccag 901 cttcacagcc ccgtccagag tctaagcctg aatctcagat tccaccacaa agacctcagc 961 gggatcaaag agtgcgagaa caacgaataa atattcctcc ccaaagggga cccagaccaa 1021 tccgtgaggc tggtgagcaa ggtgacattg aaccccgaag aatggtgaga caccctgaca 1081 gtcaccaact cttcattggc aacctgcctc atgaagtgga caaatcagag cttaaagatt 1141 tctttcaaag ttatggaaac gtggtggagt tgcgcattaa cagtggtggg aaattacCCA 1201 ATTTTGGTTT TGTTGTGTTT GATGATTCTG AGCCTGTTCA GAAAGTCCTT AGCAACAGGC 1261 CCATCATGTT CAGAGGTGAG GTCCGTCTGA ATGTCGAAGA GAAGAAGACT CGAGCTGCCA 1321 GGGAAGGCGA CCGACGAGAT AATCGCCTTC GGGGACCTGG AGGCCCTCGA GGTGGGCTGG 1381 GTGGTGGAAT GAGAGGCCCT CCCCGTGGAG GCATGGTGCA GAAACCAGGA TTTGGAGTGG 1441 GAAGGGGGCT TGCGCCACGG CAGTGCCCAA CTTTCTTGTA CAAAGTGGTT GATATCGGTA 1501 AGCCTATCCC TAACCCTCTC CTCGGTCTCG ATTCTACGTA GTAATGAACT AGTCCGTAAC 1561 TTGAAAGTAT TTCGATTTCT TGGCTTTATA TATCTTGTGG AAAGGACGAT AGACTACAAC 1621 TTGTACGTGC AATACGCGTT AAGTCgacaa tcaacctctg gattacaaaa tttgtgaaag 1681 att