Construct: ORF TRCN0000470101
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017301.1_s317c1
- Derived from:
- ccsbBroadEn_04883
- DNA Barcode:
- GAGCACTGATTAGAACTTCAGTGG
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- EMB (133418)
Vector Information:
- Vector Backbone:
- pLX_317
- Pol II Cassette 1:
- SV40-PuroR
- Pol II Cassette 2:
- EF1a-TRCN0000470101
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 133418 | EMB | embigin | NM_198449.3 | 100% | 100% | |
| 2 | human | 133418 | EMB | embigin | XM_011543146.2 | 84.7% | 84.7% | 0_1ins150 |
| 3 | human | 647121 | EMBP1 | embigin pseudogene 1 | NR_003955.1 | 20.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1047
- ORF length:
- 981
- Sequence:
-
1 tcttccattt caggtgtcgt gaggctagca tcgattgatc aacaagtttg tacaaaaaag 61 ttggcatgcg cgccctcccc ggcctgctgg aggccagggc gcgtacgccc cggctgctcc 121 tcctccagtg ccttctcgct gccgcgcgcc caagctcggc ggacggcagt gccccagatt 181 cgccttttac aagtccacct ctcagagaag aaataatggc aaataacttt tccttggaga 241 gtcataacat atcactgact gaacattcta gtatgccagt agaaaaaaat atcactttag 301 aaaggccttc taatgtaaat ctcacatgcc agttcacaac atctggggat ttgaatgcag 361 taaatgtgac ttggaaaaaa gatggtgaac aacttgagaa taattatctt gtcagtgcaa 421 caggaagcac cttgtatacc caatacaggt tcaccatcat taatagcaaa caaatgggaa 481 gttattcttg tttctttcga gaggaaaagg aacaaagggg aacatttaat ttcaaagtcc 541 ctgaacttca tgggaaaaaC AAGCCATTGA TCTCTTACGT AGGGGATTCT ACTGTCTTGA 601 CATGTAAATG TCAAAATTGT TTTCCTTTAA ATTGGACCTG GTACAGTAGT AATGGGAGTG 661 TAAAGGTTCC TGTTGGTGTT CAAATGAATA AATATGTGAT CAATGGAACA TATGCTAACG 721 AAACAAAGCT GAAGATAACA CAACTTTTGG AGGAAGATGG GGAATCTTAC TGGTGCCGTG 781 CACTATTCCA ATTAGGCGAG AGTGAAGAAC ACATTGAGCT TGTGGTGCTG AGCTATTTGG 841 TGCCCCTCAA ACCATTTCTT GTAATAGTGG CTGAGGTGAT TCTTTTAGTG GCCACCATTC 901 TGCTTTGTGA AAAGTACACA CAAAAGAAAA AGAAGCACTC AGATGAGGGG AAAGAATTTG 961 AGCAGATTGA ACAGCTGAAA TCAGATGATA GCAATGGTAT AGAAAATAAT GTCCCCAGGC 1021 ATAGAAAAAA TGAGTCTCTG GGCCAGTGCC CAACTTTCTT GTACAAAGTG GTTGATATCG 1081 GTAAGCCTAT CCCTAACCCT CTCCTCGGTC TCGATTCTAC GTAGTAATGA ACTAGTCCGT 1141 AACTTGAAAG TATTTCGATT TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAGAGCACTG 1201 ATTAGAACTT CAGTGGACGC GTTAAGTCga caatcaacct ctggattaca aaatttgtga 1261 aagatt